Application | Comment | Organism |
---|---|---|
analysis | assay of synthase and hemisynthase activities of glucosamine-6-phosphate synthase by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, allowing determination of both hemisynthase and synthase parameters from a single assay kinetic experiment | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-glutamine | synthase activity, pH 7.2, 37°C | Escherichia coli | |
0.14 | - |
L-glutamine | hemisynthase activity, pH 7.2, 37°C | Escherichia coli | |
0.5 | - |
D-fructose 6-phosphate | synthase activity, pH 7.2, 37°C | Escherichia coli | |
0.8 | - |
D-fructose 6-phosphate | hemisynthase activity, pH 7.2, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P17169 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Escherichia coli | L-glutamate + D-glucosamine 6-phosphate | - |
? | |
additional information | enzyme follows an ordered process to bind sequentially Fru-6P and L-Gln and successively releases L-glutamate (hemisynthase activity) and D-glucosamine 6-phosphate (synthase activity) | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlmS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.4 | - |
D-fructose 6-phosphate | hemisynthase activity, pH 7.2, 37°C | Escherichia coli | |
16.8 | - |
L-glutamine | hemisynthase activity, pH 7.2, 37°C | Escherichia coli | |
16.9 | - |
D-fructose 6-phosphate | synthase activity, pH 7.2, 37°C | Escherichia coli | |
16.95 | - |
L-glutamine | synthase activity, pH 7.2, 37°C | Escherichia coli |