Literature summary for 2.6.1.16 extracted from

Ghosh, S.; Blumenthal, H.J.; Davidson, E.; Roseman, S.
Glucosamine metabolism.V. Enzymatic synthesis of glucosamine 6-phosphate (1960), J. Biol. Chem., 235, 1265-1273.

Search for more literature for 2.6.1.16

General Stability

General Stability	Organism
inactivated by freezing and thawing	Escherichia coli
inactivated when exposed to a solution of pH 5.5 or below	Escherichia coli
inactivated when exposed to a solution of pH 5.5 or below	Rattus norvegicus
inactivated when exposed to a solution of pH 5.5 or below	Neurospora crassa
very unstable	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
6-diazo-5-oxo-L-norleucine	-	Escherichia coli
6-diazo-5-oxo-L-norleucine	-	Neurospora crassa
6-diazo-5-oxo-L-norleucine	-	Rattus norvegicus
azaserine	weak	Escherichia coli
azaserine	-	Neurospora crassa
azaserine	weak	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.38	-	D-fructose 6-phosphate	pH 7.7, 37°C	Rattus norvegicus
0.65	-	L-glutamine	pH 7.0, 37°C	Escherichia coli
0.75	-	D-fructose 6-phosphate	pH 7.0, 37°C	Neurospora crassa
1.6	-	L-glutamine	pH 7.7, 37°C	Rattus norvegicus
2	-	D-fructose 6-phosphate	pH 7.0, 37°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Aspergillus flavus	-	-	-
Aspergillus parasiticus	-	-	-
Colletotrichum gloeosporioides	-	-	-
Escherichia coli	-	-	-
Helminthosporium sativum	-	-	-
Hypomyces rosellus	-	-	-
Neurospora crassa	-	-	-
Neurospora tetrasperma	-	-	-
Penicillium chrysogenum	-	-	-
Penicillium sp.	-	-	-
Rattus norvegicus	-	rat	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-
liver	-	Penicillium chrysogenum	-
liver	-	Aspergillus flavus	-
liver	-	Colletotrichum gloeosporioides	-
liver	-	Aspergillus parasiticus	-
liver	-	Penicillium sp.	-
liver	-	Hypomyces rosellus	-
liver	-	Helminthosporium sativum	-
liver	-	Neurospora tetrasperma	-

Storage Stability

Storage Stability	Organism
-18°C, little loss of activity after 1 week for lyophilized material after calcium phosphate gel treatment	Neurospora crassa
0-2°C, potassium phosphate buffer, pH 7.0, 0.06 M L-glutamine, 0.01 M EDTA, stable for up to 3 or 4 weeks	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + D-fructose 6-phosphate	-	Penicillium chrysogenum	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Aspergillus flavus	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Colletotrichum gloeosporioides	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Aspergillus parasiticus	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Penicillium sp.	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Hypomyces rosellus	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Helminthosporium sativum	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	-	Neurospora tetrasperma	L-glutamate + D-glucosamine 6-phosphate	-	?
L-glutamine + D-fructose 6-phosphate	specific for: L-glutamine	Escherichia coli	L-glutamate + D-glucosamine 6-phosphate	-	ir
L-glutamine + D-fructose 6-phosphate	specific for: L-glutamine	Rattus norvegicus	L-glutamate + D-glucosamine 6-phosphate	-	ir
L-glutamine + D-fructose 6-phosphate	specific for: L-glutamine	Neurospora crassa	L-glutamate + D-glucosamine 6-phosphate	-	ir

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.7	-	-	Neurospora crassa
7.7	-	assay at	Escherichia coli
7.7	-	assay at	Rattus norvegicus
7.7	7.9	-	Rattus norvegicus
7.9	-	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no cofactor requirement	Escherichia coli
additional information	no cofactor requirement	Rattus norvegicus
additional information	no cofactor requirement	Neurospora crassa

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Release 2023.1 (January 2023)