Crystallization (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Methyl-2-benzothiazolone hydrazone hydrochloride | fully reversed by addition of pyridoxal phosphate | Lysinibacillus sphaericus | |
beta-Lysine | 80% inhibition at 10 mM after 20 min | Lysinibacillus sphaericus | |
cadaverine | fully reversed by addition of pyridoxal phosphate, 57% inhibition at 10 mM after 20 min | Lysinibacillus sphaericus | |
carboxylmethoxyamine | fully reversed by addition of pyridoxal phosphate | Lysinibacillus sphaericus | |
hydroxylamine | - |
Lysinibacillus sphaericus | |
L-arginine | fully reversed by addition of pyridoxal phosphate, 28% inhibition at 10 mM after 20 min | Lysinibacillus sphaericus | |
L-Cycloserine | fully reversed by addition of pyridoxal phosphate | Lysinibacillus sphaericus | |
L-leucine | fully reversed by addition of pyridoxal phosphate | Lysinibacillus sphaericus | |
L-lysine | 35% inhibition at 10 mM after 20 min | Lysinibacillus sphaericus | |
L-valine | fully reversed by addition of pyridoxal phosphate, 35% inhibition at 10 mM after 20 min | Lysinibacillus sphaericus | |
phenylhydrazine | fully reversed by addition of pyridoxal phosphate | Lysinibacillus sphaericus | |
putrescine | fully reversed by addition of pyridoxal phosphate, 63% inhibition at 10 mM after 20 min | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
80000 | 85000 | sedimentation equilibrium method, gel filtration | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + 2-oxoglutarate | Lysinibacillus sphaericus | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | Lysinibacillus sphaericus IFO3525 | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erwinia aroidea | - |
- |
- |
Lysinibacillus sphaericus | - |
IFO3525 | - |
Lysinibacillus sphaericus IFO3525 | - |
IFO3525 | - |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | - |
- |
Staphylococcus aureus |
0.67 | - |
- |
Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-lysine + 2-oxoglutarate | weak | Lysinibacillus sphaericus | glutamate + 3-amino-7-oxooctanoic acid | - |
? | |
beta-lysine + 2-oxoglutarate | weak | Lysinibacillus sphaericus IFO3525 | glutamate + 3-amino-7-oxooctanoic acid | - |
? | |
L-lysine + 2-oxoglutarate | weak | Lysinibacillus sphaericus | glutamate + 2-amino-7-oxooctanoic acid | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Staphylococcus aureus | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Lysinibacillus sphaericus | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Erwinia aroidea | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Lysinibacillus sphaericus | DELTA1-pyrroline-5-carboxylate + L-glutamate | glutamate-gamma semialdehyde spontaneously cyclizes to form DELTA1-pyrroline-5-carboxylate | ? | |
L-ornithine + 2-oxoglutarate | - |
Lysinibacillus sphaericus IFO3525 | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Lysinibacillus sphaericus IFO3525 | DELTA1-pyrroline-5-carboxylate + L-glutamate | glutamate-gamma semialdehyde spontaneously cyclizes to form DELTA1-pyrroline-5-carboxylate | ? | |
additional information | specific for 2-oxoglutarate | Lysinibacillus sphaericus | ? | - |
? | |
additional information | specific for ornithine | Lysinibacillus sphaericus | ? | - |
? | |
additional information | specific for 2-oxoglutarate | Lysinibacillus sphaericus IFO3525 | ? | - |
? | |
additional information | specific for ornithine | Lysinibacillus sphaericus IFO3525 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | activates | Lysinibacillus sphaericus | |
pyridoxal 5'-phosphate | 1 mol per mol of enzyme | Lysinibacillus sphaericus | |
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Lysinibacillus sphaericus | |
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Erwinia aroidea | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate is bound through an aldimine linkage to the epsilon-amino group of a lysine residue | Lysinibacillus sphaericus |