Crystallization (Comment) | Organism |
---|---|
2.25 A crystal structure of PabB that features the 273PIAGT277 motif engaged in several interactions that hold the enzyme in a distorted conformation in the absence of substrate | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
chorismate | pH 8, 25°C | Stenotrophomonas maltophilia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | one magnesium ion identified in the PabB structure, located outside of the active site | Stenotrophomonas maltophilia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-amino-4-deoxychorismate + NH3 | Stenotrophomonas maltophilia | - |
4-amino-4-deoxychorismate + NH3 | - |
? | |
(2S)-2-amino-4-deoxychorismate + NH3 | Stenotrophomonas maltophilia K279a | - |
4-amino-4-deoxychorismate + NH3 | - |
? | |
2 L-glutamine + 2 H2O | Stenotrophomonas maltophilia | - |
2 L-glutamate + 2 NH3 | - |
? | |
2 L-glutamine + 2 H2O | Stenotrophomonas maltophilia K279a | - |
2 L-glutamate + 2 NH3 | - |
? | |
chorismate + 2 L-glutamine + H2O | Stenotrophomonas maltophilia | - |
4-amino-4-deoxychorismate + 2 L-glutamate + ammonia | - |
? | |
chorismate + 2 L-glutamine + H2O | Stenotrophomonas maltophilia K279a | - |
4-amino-4-deoxychorismate + 2 L-glutamate + ammonia | - |
? | |
chorismate + NH3 | Stenotrophomonas maltophilia | - |
(2S)-2-amino-4-deoxychorismate + H2O | - |
? | |
chorismate + NH3 | Stenotrophomonas maltophilia K279a | - |
(2S)-2-amino-4-deoxychorismate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | B2FR92 | subunit PabB | - |
Stenotrophomonas maltophilia K279a | B2FR92 | subunit PabB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-amino-4-deoxychorismate + NH3 | - |
Stenotrophomonas maltophilia | 4-amino-4-deoxychorismate + NH3 | - |
? | |
(2S)-2-amino-4-deoxychorismate + NH3 | - |
Stenotrophomonas maltophilia K279a | 4-amino-4-deoxychorismate + NH3 | - |
? | |
2 L-glutamine + 2 H2O | - |
Stenotrophomonas maltophilia | 2 L-glutamate + 2 NH3 | - |
? | |
2 L-glutamine + 2 H2O | - |
Stenotrophomonas maltophilia K279a | 2 L-glutamate + 2 NH3 | - |
? | |
chorismate + 2 L-glutamine + H2O | - |
Stenotrophomonas maltophilia | 4-amino-4-deoxychorismate + 2 L-glutamate + ammonia | - |
? | |
chorismate + 2 L-glutamine + H2O | - |
Stenotrophomonas maltophilia K279a | 4-amino-4-deoxychorismate + 2 L-glutamate + ammonia | - |
? | |
chorismate + NH3 | - |
Stenotrophomonas maltophilia | (2S)-2-amino-4-deoxychorismate + H2O | - |
? | |
chorismate + NH3 | - |
Stenotrophomonas maltophilia K279a | (2S)-2-amino-4-deoxychorismate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49500, calculated from sequence | Stenotrophomonas maltophilia |
Synonyms | Comment | Organism |
---|---|---|
PabB | - |
Stenotrophomonas maltophilia |
Smlt1032 | - |
Stenotrophomonas maltophilia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
chorismate | overall reaction, pH 8, 25°C | Stenotrophomonas maltophilia | |
0.02 | - |
chorismate | second reaction step, cosubstrate NH3, pH 8, 25°C | Stenotrophomonas maltophilia |
General Information | Comment | Organism |
---|---|---|
metabolism | PabB produces 4-amino-4-deoxychorismate via a noncovalent 2-amino-2-deoxyisochorismate intermediate. 2-amino-4-deoxyisochorismate is released into solution prior to being rebound and converted to 4-amino-4-deoxyisochorismate in a second PabB-catalyzed reaction. PabB is virtually inactive alone but forms a high affinity complex with E. coli PabA | Stenotrophomonas maltophilia |