Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
N2-Acetyl-L-ornithine | pH 8.9, 45°C | Thermus thermophilus | |
10 | - |
N2-acetyl-L-lysine | pH 8.9, 45°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q93R93 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N2-acetyl-L-lysine + 2-oxoglutarate | ping-pong bi-bi mechanism | Thermus thermophilus | N2-acetyl-L-aminoadipate semialdehyde + L-glutamate | - |
? | |
N2-acetyl-L-ornithine + 2-oxoglutarate | - |
Thermus thermophilus | (2R)-2-acetamido-5-oxopentanoic acid + L-glutamate | - |
? | |
[LysW]-aminoadipate 6-semialdehyde + L-glutamate | - |
Thermus thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE, 2 * 43503, calculated from sequence | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
lysJ | - |
Thermus thermophilus |
[LysW]-aminoadipate semialdehyde transaminase | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
N2-acetyl-L-lysine | pH 8.9, 45°C | Thermus thermophilus | |
1 | - |
N2-Acetyl-L-ornithine | pH 8.9, 45°C | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | gene product is essential for lysine biosynthesis in Thermus thermophilus | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0786 | - |
N2-acetyl-L-lysine | pH 8.9, 45°C | Thermus thermophilus | |
1.266 | - |
N2-Acetyl-L-ornithine | pH 8.9, 45°C | Thermus thermophilus |