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Literature summary for 2.6.1.118 extracted from

  • Miyazaki, J.; Kobashi, N.; Nishiyama, M.; Yamane, H.
    Functional and evolutionary relationship between arginine biosynthesis and prokaryotic lysine biosynthesis through alpha-aminoadipate (2001), J. Bacteriol., 183, 5067-5073 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
N2-Acetyl-L-ornithine pH 8.9, 45°C Thermus thermophilus
10
-
N2-acetyl-L-lysine pH 8.9, 45°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q93R93
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N2-acetyl-L-lysine + 2-oxoglutarate ping-pong bi-bi mechanism Thermus thermophilus N2-acetyl-L-aminoadipate semialdehyde + L-glutamate
-
?
N2-acetyl-L-ornithine + 2-oxoglutarate
-
Thermus thermophilus (2R)-2-acetamido-5-oxopentanoic acid + L-glutamate
-
?
[LysW]-aminoadipate 6-semialdehyde + L-glutamate
-
Thermus thermophilus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE, 2 * 43503, calculated from sequence Thermus thermophilus

Synonyms

Synonyms Comment Organism
lysJ
-
Thermus thermophilus
[LysW]-aminoadipate semialdehyde transaminase
-
Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
N2-acetyl-L-lysine pH 8.9, 45°C Thermus thermophilus
1
-
N2-Acetyl-L-ornithine pH 8.9, 45°C Thermus thermophilus

General Information

General Information Comment Organism
physiological function gene product is essential for lysine biosynthesis in Thermus thermophilus Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0786
-
N2-acetyl-L-lysine pH 8.9, 45°C Thermus thermophilus
1.266
-
N2-Acetyl-L-ornithine pH 8.9, 45°C Thermus thermophilus