Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate dependent aminotransferase | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression as a His-tagged fusion protein in Escherichia coli BL21 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
GDP-4-dehydro-6-deoxy-D-mannose | pH 7.5, 37°C | Escherichia coli | |
2.3 | - |
L-glutamate | pH 7.5, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44330 | - |
10 * 44330, His-tag PerA, SDS-PAGE | Escherichia coli |
458000 | - |
His-tag PerA, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose + L-glutamate | Escherichia coli | - |
GDP-alpha-D-perosamine + 2-oxoglutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged fusion protein | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 30 days, His-tag PerA, residual activity of 68% | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose + L-glutamate | - |
Escherichia coli | GDP-alpha-D-perosamine + 2-oxoglutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 44330, His-tag PerA, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase | - |
Escherichia coli |
PerA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
His-tag PerA holds 55% residual activity after 20 min incubation | Escherichia coli |
60 | - |
His-tag PerA, 10 min, completely inactivated | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
8 | - |
assay at | Escherichia coli |