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Literature summary for 2.6.1.102 extracted from

  • Albermann, C.; Beuttler, H.
    Identification of the GDP-N-acetyl-d-perosamine producing enzymes from Escherichia coli O157:H7 (2008), FEBS Lett., 582, 479-484.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
pyridoxal 5'-phosphate pyridoxal 5'-phosphate dependent aminotransferase Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression as a His-tagged fusion protein in Escherichia coli BL21 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
GDP-4-dehydro-6-deoxy-D-mannose pH 7.5, 37°C Escherichia coli
2.3
-
L-glutamate pH 7.5, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44330
-
10 * 44330, His-tag PerA, SDS-PAGE Escherichia coli
458000
-
His-tag PerA, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-4-dehydro-6-deoxy-D-mannose + L-glutamate Escherichia coli
-
GDP-alpha-D-perosamine + 2-oxoglutarate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged fusion protein Escherichia coli

Storage Stability

Storage Stability Organism
4°C, 30 days, His-tag PerA, residual activity of 68% Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-6-deoxy-D-mannose + L-glutamate
-
Escherichia coli GDP-alpha-D-perosamine + 2-oxoglutarate
-
?

Subunits

Subunits Comment Organism
decamer 10 * 44330, His-tag PerA, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase
-
Escherichia coli
PerA
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
His-tag PerA holds 55% residual activity after 20 min incubation Escherichia coli
60
-
His-tag PerA, 10 min, completely inactivated Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Escherichia coli
8
-
assay at Escherichia coli