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Literature summary for 2.6.1.1 extracted from

  • Rakhmanova, T.I.; Popova, T.N.; Semenikhina, A.V.
    Subcellular localization, purification, and some catalytic properties of aspartate aminotransferase from Spirodela polyrhiza (2006), Biol. Bull., 33, 347-352.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
L-aspartate cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase Spirodela polyrhiza
0.5
-
L-glutamate cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase Spirodela polyrhiza
0.58
-
L-glutamate mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase Spirodela polyrhiza
0.78
-
L-aspartate mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase Spirodela polyrhiza
1.33
-
2-oxoglutarate cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase Spirodela polyrhiza
1.95
-
2-oxoglutarate mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase Spirodela polyrhiza
2.04
-
oxaloacetate mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase Spirodela polyrhiza
2.45
-
oxaloacetate cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase Spirodela polyrhiza

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast 24% activity is localized in chloroplasts Spirodela polyrhiza 9507
-
cytoplasm 73% of total cellular content is localized in the cytoplasm Spirodela polyrhiza 5737
-
mitochondrion 2.8% activity is localized in mitochondria Spirodela polyrhiza 5739
-

Organism

Organism UniProt Comment Textmining
Spirodela polyrhiza
-
-
-
Spirodela polyrhiza SJ
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-25 gel filtration and DEAE-Toyopearl column chromatography Spirodela polyrhiza

Source Tissue

Source Tissue Comment Organism Textmining
thallus
-
Spirodela polyrhiza
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
homogenate, mitochondrial enzyme Spirodela polyrhiza
0.04
-
homogenate, cytoplasmic enzyme Spirodela polyrhiza
0.459
-
after 30fold purification, mitochondrial enzyme Spirodela polyrhiza
1.41
-
after 35fold purification, cytoplasmic enzyme Spirodela polyrhiza

Storage Stability

Storage Stability Organism
0-4°C, 50% glycerol, 1 day, remains active, (cytoplasmic enzyme) Spirodela polyrhiza
0-4°C, 50% glycerol, 3-5 h, remains active, (mitochondrial enzyme) Spirodela polyrhiza

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Spirodela polyrhiza oxaloacetate + L-glutamate
-
r
L-aspartate + 2-oxoglutarate
-
Spirodela polyrhiza SJ oxaloacetate + L-glutamate
-
r

Synonyms

Synonyms Comment Organism
AAT
-
Spirodela polyrhiza
aspartate aminotransferase
-
Spirodela polyrhiza