KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
L-aspartate | cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase | Spirodela polyrhiza | |
0.5 | - |
L-glutamate | cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase | Spirodela polyrhiza | |
0.58 | - |
L-glutamate | mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase | Spirodela polyrhiza | |
0.78 | - |
L-aspartate | mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase | Spirodela polyrhiza | |
1.33 | - |
2-oxoglutarate | cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase | Spirodela polyrhiza | |
1.95 | - |
2-oxoglutarate | mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase | Spirodela polyrhiza | |
2.04 | - |
oxaloacetate | mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase | Spirodela polyrhiza | |
2.45 | - |
oxaloacetate | cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase | Spirodela polyrhiza |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | 24% activity is localized in chloroplasts | Spirodela polyrhiza | 9507 | - |
cytoplasm | 73% of total cellular content is localized in the cytoplasm | Spirodela polyrhiza | 5737 | - |
mitochondrion | 2.8% activity is localized in mitochondria | Spirodela polyrhiza | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spirodela polyrhiza | - |
- |
- |
Spirodela polyrhiza SJ | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-25 gel filtration and DEAE-Toyopearl column chromatography | Spirodela polyrhiza |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thallus | - |
Spirodela polyrhiza | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
homogenate, mitochondrial enzyme | Spirodela polyrhiza |
0.04 | - |
homogenate, cytoplasmic enzyme | Spirodela polyrhiza |
0.459 | - |
after 30fold purification, mitochondrial enzyme | Spirodela polyrhiza |
1.41 | - |
after 35fold purification, cytoplasmic enzyme | Spirodela polyrhiza |
Storage Stability | Organism |
---|---|
0-4°C, 50% glycerol, 1 day, remains active, (cytoplasmic enzyme) | Spirodela polyrhiza |
0-4°C, 50% glycerol, 3-5 h, remains active, (mitochondrial enzyme) | Spirodela polyrhiza |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Spirodela polyrhiza | oxaloacetate + L-glutamate | - |
r | |
L-aspartate + 2-oxoglutarate | - |
Spirodela polyrhiza SJ | oxaloacetate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AAT | - |
Spirodela polyrhiza |
aspartate aminotransferase | - |
Spirodela polyrhiza |