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Literature summary for 2.6.1.1 extracted from

  • Seetharamappa, J.; Oke, M.; Liu, H.; McMahon, S.A.; Johnson, K.A.; Carter, L.; Dorward, M.; Zawadzki, M.; Overton, I.M.; van Niekirk, C.A.; Graham, S.; Botting, C.H.; Taylor, G.L.; White, M.F.; Barton, G.J.; Coote, P.J.; Naismith, J.H.
    Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5-phosphate-dependent aminotransferase (2007), Acta Crystallogr. Sect. F, F63, 452-456.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion and sitting drop vapour diffusion using 23.6% (w/v) PEG 4000, 0.1 M HEPES pH 8.0 Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48170
-
-
Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel Sepharose 6 fast flow chromatography and S-75 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Staphylococcus aureus oxaloacetate + L-glutamate
-
?

Subunits

Subunits Comment Organism
dimer
-
Staphylococcus aureus

Synonyms

Synonyms Comment Organism
aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase
-
Staphylococcus aureus
Sar2028
-
Staphylococcus aureus

pI Value

Organism Comment pI Value Maximum pI Value
Staphylococcus aureus
-
-
5.25