Cloned (Comment) | Organism |
---|---|
cloning, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli AB1157 | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Thermus thermophilus | |
additional information | - |
additional information | recombinant enzyme | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Thermus thermophilus | - |
oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from overproducing Escherichia coli AB1157 cells, 66fold | Thermus thermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | bi bi ping pong reaction kinetic | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
112.1 | - |
purified recombinant enzyme | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Thermus thermophilus | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Thermus thermophilus | oxaloacetate + L-glutamate | - |
r | |
L-aspartate + 2-oxoglutarate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | oxaloacetate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AspAT | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
high thermostability, extra prolyl residues, located at the surface of the protein, are involved | Thermus thermophilus |
additional information | - |
thermal denaturation is irreversible | Thermus thermophilus |
75 | - |
stable up to | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal 5'-phosphate protein | Thermus thermophilus | |
pyridoxal 5'-phosphate | enzyme-bound | Thermus thermophilus |