Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.1 extracted from

  • Taniguchi, M.; Sugiyama, T.
    Aspartate aminotransferase from Eleusine coracana, a C4 plant: purification, characterization, and preparation of antibody (1990), Arch. Biochem. Biophys., 282, 427-432.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
2-oxoglutarate isozyme AspAT-3, 25°C, pH 8.0 Eleusine coracana
0.2
-
oxaloacetate isozyme AspAT-1, 25°C, pH 8.0 Eleusine coracana
0.23
-
2-oxoglutarate isozyme AspAT-1, 25°C, pH 8.0 Eleusine coracana
0.32
-
oxaloacetate isozyme AspAT-3, 25°C, pH 8.0 Eleusine coracana
1.7
-
L-aspartate isozyme AspAT-3, 25°C, pH 8.0 Eleusine coracana
7.5
-
L-aspartate isozyme AspAT-1, 25°C, pH 8.0 Eleusine coracana
12.2
-
L-glutamate isozyme AspAT-3, 25°C, pH 8.0 Eleusine coracana
17.4
-
L-glutamate isozyme AspAT-1, 25°C, pH 8.0 Eleusine coracana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Eleusine coracana
80000
-
gel filtration Eleusine coracana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Eleusine coracana function in C4 acid pathway oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Eleusine coracana
-
isoenzymes: AspAT-1, AspAT-2, AspAT-3
-

Purification (Commentary)

Purification (Comment) Organism
isoenzymes: AspAT-1, AspAT-2, AspAT-3 Eleusine coracana

Source Tissue

Source Tissue Comment Organism Textmining
bundle sheath AspAT-3 Eleusine coracana
-
leaf
-
Eleusine coracana
-
mesophyll AspAT-1 Eleusine coracana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105
-
purified enzyme Eleusine coracana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Eleusine coracana oxaloacetate + L-glutamate
-
r
L-aspartate + 2-oxoglutarate function in C4 acid pathway Eleusine coracana oxaloacetate + L-glutamate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Eleusine coracana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Eleusine coracana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
formation of glutamate Eleusine coracana
8.5
-
L-aspartate formation Eleusine coracana