Cloned (Comment) | Organism |
---|---|
gene bcl-hept, DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged enzyme from pCold I vector as soluble protein in Escherichia coli strain BL21(DE3) | Alkalihalobacillus clausii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Alkalihalobacillus clausii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | Alkalihalobacillus clausii | heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | Alkalihalobacillus clausii JCM 9138 | heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | Alkalihalobacillus clausii | heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | Alkalihalobacillus clausii JCM 9138 | heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + isopentenyl diphosphate | Alkalihalobacillus clausii | heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.81 | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? | |
additional information | Alkalihalobacillus clausii | formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate | ? | - |
? | |
additional information | Alkalihalobacillus clausii JCM 9138 | formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus clausii | S6AGL5 | gene bcl-hept | - |
Alkalihalobacillus clausii | S6B6P7 | gene bcl-hept | - |
Alkalihalobacillus clausii JCM 9138 | S6AGL5 | gene bcl-hept | - |
Alkalihalobacillus clausii JCM 9138 | S6B6P7 | gene bcl-hept | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Alkalihalobacillus clausii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate | (3) | Alkalihalobacillus clausii | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate = 2 diphosphate + all-trans-hexaprenyl diphosphate | (2) | Alkalihalobacillus clausii | |
geranylgeranyl diphosphate + isopentenyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | (1) | Alkalihalobacillus clausii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity | Alkalihalobacillus clausii | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30 | Alkalihalobacillus clausii | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity | Alkalihalobacillus clausii JCM 9138 | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30 | Alkalihalobacillus clausii JCM 9138 | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 | Alkalihalobacillus clausii | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 | Alkalihalobacillus clausii JCM 9138 | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + isopentenyl diphosphate | heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.81 | Alkalihalobacillus clausii | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? | |
additional information | formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate | Alkalihalobacillus clausii | ? | - |
? | |
additional information | product identification and determination by mass spectrometry, NMR spectrometry, and TLC | Alkalihalobacillus clausii | ? | - |
? | |
additional information | formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate | Alkalihalobacillus clausii JCM 9138 | ? | - |
? | |
additional information | product identification and determination by mass spectrometry, NMR spectrometry, and TLC | Alkalihalobacillus clausii JCM 9138 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(all-E)-isoprenyl diphosphate synthase | - |
Alkalihalobacillus clausii |
E-IDS2-L | - |
Alkalihalobacillus clausii |
E-IDS2-S | - |
Alkalihalobacillus clausii |
geranylfarnesyl diphosphate/hexaprenyl diphosphate/heptaprenyl diphosphate synthase | - |
Alkalihalobacillus clausii |
GFPP/HexPP/HepPP synthase | - |
Alkalihalobacillus clausii |
HepS | - |
Alkalihalobacillus clausii |
HepT | - |
Alkalihalobacillus clausii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Alkalihalobacillus clausii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Alkalihalobacillus clausii |
General Information | Comment | Organism |
---|---|---|
metabolism | hexaprenyl diphosphate and heptaprenyl diphosphate, intermediates of two isoprenoid pathways (acyclic terpenes and menaquinones), are biosynthesized by one trifunctional (all-E)-isoprenyl diphosphate synthase, E-IDS. Geranylfarnesyl diphosphate/hexaprenyl diphosphate and heptaprenyl diphosphate are the primary substrates for the biosynthesis of acyclic terpenes and menaquinone-7, respectively. (All-E)-and E,Z-mixed isoprenyl diphosphates are formed by E-IDSs and Z-IDSs, respectively, which have no sequence similarity with each other | Alkalihalobacillus clausii |
physiological function | all-trans-heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), and is used to produce menaquinone-7 | Alkalihalobacillus clausii |