Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli coenzyme Q8 biosynthesis-deficient strains, HW108 and HW109, which contain mutations in ubiE and ubiG, respectively. Gene lePGT-1 gene is amplified and cloned into the expression vector, pBAD33, to form pBlePGT, the fine-tuning of the expression of lePGT-1 in the ubiquinone-deficient strains, HW108 and HW109, to control the electron transfer chain is investigated by adding different concentrations of the inducer arabinose at 0-20 mM under fully aerobic conditions | Lithospermum erythrorhizon |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Lithospermum erythrorhizon | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + 4-hydroxybenzoate | Lithospermum erythrorhizon | - |
3-geranyl-4-hydroxybenzoate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lithospermum erythrorhizon | Q8W405 | gene PGT-1 or lepgt | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + 4-hydroxybenzoate | - |
Lithospermum erythrorhizon | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
diphosphate:4-hydroxybenzoate geranyltransferase | - |
Lithospermum erythrorhizon |
geranyl diphosphate:4-hydroxybenzoate 3-geranyltransferase | - |
Lithospermum erythrorhizon |
lepgt | - |
Lithospermum erythrorhizon |
PGT-1 | - |
Lithospermum erythrorhizon |
General Information | Comment | Organism |
---|---|---|
metabolism | the reaction catalyzed by lePGT-1 plays as a competing pathway on two substrates of the Q8 synthesis pathway, namely at IPP and 4-hydroxybenzoate | Lithospermum erythrorhizon |
physiological function | the enzyme is part of a metabolic strategy to further controll the respiratory levels in CoQ8 biosynthesis-deficient Escherichia coli strains, this metabolic strategy can lead to fine-tuning of aerobic lactate accumulation. The lactate and acetate concentrations increase with increasing enzyme levels, as does the cell density, while glucose concentration decreases, overview | Lithospermum erythrorhizon |