Literature summary for 2.5.1.90 extracted from

Li, X.; Han, X.; Ko, T.P.; Chen, C.C.; Zhu, Z.; Hua, E.; Guo, R.T.; Huang, C.H.
Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli (2013), Acta Crystallogr. Sect. F, 69, 328-331.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21trxB (DE3) from vector pET46 Ek/LIC	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, crystallization method screening, sitting drop vapour diffusion method, mixing of 0.002 ml of 3 mg/ml protein in 25 mM Tris-HCl, 150 mM NaCl, pH 7.5, with 0.002 ml of crystallization solution containing 0.3 M magnesium chloride hexahydrate, 0.1 M Tris-HCl, pH 8.5, 24% w/v PEG 3350, 3-4 days, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement, the crystal contains one homodimer per asymmetric unit, structure modelling	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant enzyme, crystallization method screening, sitting drop vapour diffusion method, mixing of 0.002 ml of 3 mg/ml protein in 25 mM Tris-HCl, 150 mM NaCl, pH 7.5, with 0.002 ml of crystallization solution containing 0.3 M magnesium chloride hexahydrate, 0.1 M Tris-HCl, pH 8.5, 24% w/v PEG 3350, 3-4 days, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement, the crystal contains one homodimer per asymmetric unit, structure modelling

Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate	Escherichia coli	the enzyme catalyzes the successive condensation of farnesyl diphosphate with five isopentenyl pyrophosphate molecules to form trans-C40-octaprenyl diphosphate	5 diphosphate + all-trans-octaprenyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AD57	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21trxB (DE3) by nickel affinity and anion exchange chromatography, dialysis, and ultrafiltration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate	-	Escherichia coli	5 diphosphate + all-trans-octaprenyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate	the enzyme catalyzes the successive condensation of farnesyl diphosphate with five isopentenyl pyrophosphate molecules to form trans-C40-octaprenyl diphosphate	Escherichia coli	5 diphosphate + all-trans-octaprenyl diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
octaprenyl pyrophosphate synthase	-	Escherichia coli
Opp	-	Escherichia coli

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the E-type prenyltransferase family	Escherichia coli
additional information	enzyme structure modelling using the structure of Rhodobacter capsulatus decaprenyl diphosphate synthase, PDB ID 3mzv, as a template model	Escherichia coli
physiological function	the enzyme is essential for the biosynthesis of bacterial ubiquinone or menaquinone side chains, which play an important role in the electron-transport system	Escherichia coli