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Literature summary for 2.5.1.90 extracted from

  • Chang, K.; Chen, S.; Kuo, C.; Chang, C.; Guo, R.; Yang, J.; Liang, P.
    Roles of amino acids in the Escherichia coli octaprenyl diphosphate synthase active site probed by structure-guided site-directed mutagenesis (2012), Biochemistry, 51, 3412-3419.
    View publication on PubMed
Search for more literature for 2.5.1.90

Protein Variants

Protein Variants Comment Organism
D211A 0.37% of wild-type activity Escherichia coli
D212A 0.091% of wild-type activity Escherichia coli
D215A 0.43% of wild-type activity Escherichia coli
D84A 0.006% of wild-type activity Escherichia coli
D85A 0.016% of wild-type activity Escherichia coli
D88A 0.0057% of wild-type activity Escherichia coli
H77A 0.95% of wild-type activity Escherichia coli
K170L 0.017% of wild-type activity Escherichia coli
K225L 0.45% of wild-type activity Escherichia coli
K235L 0.096% of wild-type activity Escherichia coli
K45A 0.18% of wild-type activity Escherichia coli
additional information residue D85 is the most important residue in the first DDXXD motif for both farnesyl diphosphate and isopentenyl diphosphate binding through an H-bond network involving R93 and R94, respectively, whereas R94, K45, R48, and H77 are responsible for isopentenyl binding by providing H-bonds and ionic interactions. K170 and T171 may stabilize the farnesyl carbocation intermediate to facilitate the reaction, whereas R93 and K225 may stabilize the catalytic base for HR proton abstraction after isopentenyl diphosphate condensation. K225 and K235 in a flexible loop may interact with farnesyl diphosphate when the enzyme becomes a closed conformation, which is therefore crucial for catalysis. Q208 is near the hydrophobic part of isopentenyl diphosphate and is important for isopentenyl diphosphate binding and catalysis Escherichia coli
Q208A 0.048% of wild-type activity Escherichia coli
R48A 0.057% of wild-type activity Escherichia coli
R93A 0.0058% of wild-type activity Escherichia coli
R94A 0.0042% of wild-type activity Escherichia coli
T171V 0.22% of wild-type activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 (2E,6E)-farnesyl diphosphate mutant K225L, pH 7.5, 25°C Escherichia coli
0.0008
-
 (2E,6E)-farnesyl diphosphate mutant Q208A, pH 7.5, 25°C Escherichia coli
0.001
-
 (2E,6E)-farnesyl diphosphate mutant K45A, pH 7.5, 25°C Escherichia coli
0.0012
-
 (2E,6E)-farnesyl diphosphate mutant T171V, pH 7.5, 25°C Escherichia coli
0.0015
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 25°C Escherichia coli
0.0015
-
 (2E,6E)-farnesyl diphosphate mutant D215A, pH 7.5, 25°C Escherichia coli
0.0017
-
 (2E,6E)-farnesyl diphosphate mutant D211A, pH 7.5, 25°C Escherichia coli
0.0018
-
 (2E,6E)-farnesyl diphosphate mutant K235L, pH 7.5, 25°C Escherichia coli
0.0024
-
 isopentenyl diphosphate mutant Q208A, pH 7.5, 25°C Escherichia coli
0.0028
-
 (2E,6E)-farnesyl diphosphate mutant R94A, pH 7.5, 25°C Escherichia coli
0.0031
-
 (2E,6E)-farnesyl diphosphate mutant D212A, pH 7.5, 25°C Escherichia coli
0.0032
-
 (2E,6E)-farnesyl diphosphate mutant K170L, pH 7.5, 25°C Escherichia coli
0.0037
-
 isopentenyl diphosphate mutant R93A, pH 7.5, 25°C Escherichia coli
0.0038
-
 (2E,6E)-farnesyl diphosphate mutant D88A, pH 7.5, 25°C Escherichia coli
0.004
-
 isopentenyl diphosphate wild-type, pH 7.5, 25°C Escherichia coli
0.0045
-
 isopentenyl diphosphate mutant T171V, pH 7.5, 25°C Escherichia coli
0.0049
-
 isopentenyl diphosphate mutant K170L, pH 7.5, 25°C Escherichia coli
0.0052
-
 (2E,6E)-farnesyl diphosphate mutant R93A, pH 7.5, 25°C Escherichia coli
0.0085
-
 isopentenyl diphosphate mutant D88A, pH 7.5, 25°C Escherichia coli
0.0092
-
 (2E,6E)-farnesyl diphosphate mutant D84A, pH 7.5, 25°C Escherichia coli
0.0093
-
 isopentenyl diphosphate mutant D84A, pH 7.5, 25°C Escherichia coli
0.0113
-
 isopentenyl diphosphate mutant K225L, pH 7.5, 25°C Escherichia coli
0.0182
-
 isopentenyl diphosphate mutant D215A, pH 7.5, 25°C Escherichia coli
0.0233
-
 isopentenyl diphosphate mutant D212A, pH 7.5, 25°C Escherichia coli
0.024
-
 (2E,6E)-farnesyl diphosphate mutant D85A, pH 7.5, 25°C Escherichia coli
0.0242
-
 (2E,6E)-farnesyl diphosphate mutant H77A, pH 7.5, 25°C Escherichia coli
0.0246
-
 (2E,6E)-farnesyl diphosphate mutant R48A, pH 7.5, 25°C Escherichia coli
0.0271
-
 isopentenyl diphosphate mutant D211A, pH 7.5, 25°C Escherichia coli
0.029
-
 isopentenyl diphosphate mutant D85A, pH 7.5, 25°C Escherichia coli
0.0335
-
 isopentenyl diphosphate mutant K45A, pH 7.5, 25°C Escherichia coli
0.0539
-
 isopentenyl diphosphate mutant R94A, pH 7.5, 25°C Escherichia coli
0.0639
-
 isopentenyl diphosphate mutant R48A, pH 7.5, 25°C Escherichia coli
0.0745
-
 isopentenyl diphosphate mutant K235L, pH 7.5, 25°C Escherichia coli
0.102
-
 isopentenyl diphosphate mutant H77A, pH 7.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AD57
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000084
-
 (2E,6E)-farnesyl diphosphate mutant R94A, pH 7.5, 25°C Escherichia coli
0.00011
-
 (2E,6E)-farnesyl diphosphate mutant D88A, pH 7.5, 25°C Escherichia coli
0.00012
-
 (2E,6E)-farnesyl diphosphate mutant D84A, pH 7.5, 25°C Escherichia coli
0.00012
-
 (2E,6E)-farnesyl diphosphate mutant R93A, pH 7.5, 25°C Escherichia coli
0.00032
-
 (2E,6E)-farnesyl diphosphate mutant D85A, pH 7.5, 25°C Escherichia coli
0.00033
-
 (2E,6E)-farnesyl diphosphate mutant K170L, pH 7.5, 25°C Escherichia coli
0.0011
-
 (2E,6E)-farnesyl diphosphate mutant R48A, pH 7.5, 25°C Escherichia coli
0.0018
-
 (2E,6E)-farnesyl diphosphate mutant D212A, pH 7.5, 25°C Escherichia coli
0.0019
-
 (2E,6E)-farnesyl diphosphate mutant K235L, pH 7.5, 25°C Escherichia coli
0.0036
-
 (2E,6E)-farnesyl diphosphate mutant K45A, pH 7.5, 25°C Escherichia coli
0.0043
-
 (2E,6E)-farnesyl diphosphate mutant T171V, pH 7.5, 25°C Escherichia coli
0.0075
-
 (2E,6E)-farnesyl diphosphate mutant D211A, pH 7.5, 25°C Escherichia coli
0.0087
-
 (2E,6E)-farnesyl diphosphate mutant D215A, pH 7.5, 25°C Escherichia coli
0.0091
-
 (2E,6E)-farnesyl diphosphate mutant K225L, pH 7.5, 25°C Escherichia coli
0.0096
-
 (2E,6E)-farnesyl diphosphate mutant Q208A, pH 7.5, 25°C Escherichia coli
0.019
-
 (2E,6E)-farnesyl diphosphate mutant H77A, pH 7.5, 25°C Escherichia coli
2
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 25°C Escherichia coli