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Literature summary for 2.5.1.90 extracted from
- Reaction kinetic pathway of the recombinant octaprenyl pyrophosphate synthase from Thermotoga maritima: how is it different from that of the mesophilic enzyme (2002), Biochim. Biophys. Acta, 1599, 125-133.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Thermotoga maritima |  |
| 0.002 | - |
isopentenyl diphosphate | pH 7.5, 25°C | Thermotoga maritima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-octaprenyl diphosphate | isopentenyl diphosphate condensation rather than product release is rate limiting | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Thermotoga maritima | 5 diphosphate + all-trans-octaprenyl diphosphate | the product of chain elongation is C40 and the rate of its conversion to C45 is negligible. Under single-turnover condition with 0.01 mM enzyme-farnesyl diphosphate complex and 0.001 mM isopentenyl diphosphate, only the C20 is formed | ? | |
| additional information | the isopentenyl diphosphate condensation represents the rate-limiting step of the reaction | Thermotoga maritima | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
melting temeprature is abve 80°C | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Thermotoga maritima | |
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