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Literature summary for 2.5.1.9 extracted from
- Evolution of vitamin B2 biosynthesis. A novel class of riboflavin synthase in Archaea (2004), J. Mol. Biol., 343, 267-278.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| optimization of expression in Escherichia coli by construction of a synthetic gene | Methanocaldococcus jannaschii |
| optimization of expression in Escherichia coli by construction of a synthetic gene, phylogenetic analysis | Methanocaldococcus jannaschii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | preferred cation | Methanocaldococcus jannaschii |  |
| Mn2+ | preferred cation | Methanocaldococcus jannaschii | |
| additional information | divalent cations are required for maximal activity | Methanocaldococcus jannaschii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17500 | - |
5 * 17500, recombinant enzyme | Methanocaldococcus jannaschii |
| 88300 | - |
sedimentation equilibrium centrifugation, recombinant enzyme | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6,7-dimethyl-8-(1-D-ribityl)lumazine | Methanocaldococcus jannaschii | ultimate step in the biosynthesis of riboflavin, pathway overview, evolution of vitamin B2 biosynthesis | riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
AY442958, GenBank synthetic gene | - |
| Methanocaldococcus jannaschii | Q58584 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli to homogeneity | Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.002 | - |
purified recombinant enzyme, in absence of divalent cations | Methanocaldococcus jannaschii |
| 0.024 | - |
purified recombinant enzyme, in presence of divalent cations | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6,7-dimethyl-8-(1-D-ribityl)lumazine | ultimate step in the biosynthesis of riboflavin, pathway overview, evolution of vitamin B2 biosynthesis | Methanocaldococcus jannaschii | riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione | - |
? | |
| 6,7-dimethyl-8-(1-D-ribityl)lumazine | transfer of a 4-carbon fragment | Methanocaldococcus jannaschii | riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione | - |
? | |
| additional information | activity with different isotopomers, stereochemistry, overview | Methanocaldococcus jannaschii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| pentamer | 5 * 17500, recombinant enzyme | Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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