Literature summary for 2.5.1.9 extracted from

Kis, K.; Bacher, A.
Substrate channeling in the lumazine synthase/riboflavin synthase complex of Bacillus subtilis (1995), J. Biol. Chem., 270, 16788-16795.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	sigmoidal kinetics is observed with mixtures of artifactual beta 60 capsids and alpha subunit trimers. Km value for lumazine of the lumazine synthase/riboflavin synthase complex is about 10 times larger as compared to light riboflavin synthase consisting of alpha subunits, without lumazine synthase activity	Bacillus subtilis
0.13	-	6,7-dimethyl-8-(1'-D-ribityl)lumazine	pH 7.0, 37°C	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	lumazine synthase/riboflavin synthase complex, the alpha subunit carries riboflavin synthase activity	-

Reaction

Reaction	Comment	Organism	Reaction ID
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	formation of riboflavin by the lumazine synthase/riboflavin synthase complex follows a leaky channel model	Bacillus subtilis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine	-	Bacillus subtilis	riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione	catalysed by the alpha-subunit, which forms a trimer surrounded by an icosahedral capsid of 60 beta subunits producing substrate 6,7-dimethyl-8-(1-D-ribityl)lumazine	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.019	-	6,7-dimethyl-8-ribityllumazine	pH 7.0, 37°C	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	both lumazine synthase and riboflavin synthase activity	Bacillus subtilis

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Release 2023.1 (January 2023)