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Literature summary for 2.5.1.87 extracted from
- Molecular cloning, expression, and functional analysis of a cis-prenyltransferase from Arabidopsis thaliana. Implications in rubber biosynthesis (2000), J. Biol. Chem., 275, 18482-18488.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | not strictly dependent on TRiton X-100, maximum activity at 0.01% Triton X-100 | Arabidopsis thaliana |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | no activity in absence of Mg2+. Rapid increase of enzyme activity with the addition of Mg2+ up 2 mM. Further addition of Mg2+ inhibits the activity | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00013 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C | Arabidopsis thaliana | |
| 0.00362 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Arabidopsis thaliana | |
| 0.023 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | no activity in absence of Mg2+. Rapid increase of enzyme activity with the addition of Mg2+ up 2 mM. Further addition of Mg2+ inhibits the activity | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, calculated from sequence | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 21 isopentenyl diphosphate | Arabidopsis thaliana | trans,trans-farnesyl diphosphate is a better substrate than geranylgeranyl diphosphate. The enzyme catalyzes the formation of polyprenyl diphosphates with predominant carbon number C120. In vitro rubber biosynthesis analysis indicates that the Arabidopsis cis-prenyltransferase itself could not catalyze the formation of high molecular weight polyprenyl diphosphate such as natural rubber | 21 diphosphate + di-trans,poly-cis tetracosaprenyl diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O80458 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 21 isopentenyl diphosphate | trans,trans-farnesyl diphosphate is a better substrate than geranylgeranyl diphosphate. The enzyme catalyzes the formation of polyprenyl diphosphates with predominant carbon number C120. In vitro rubber biosynthesis analysis indicates that the Arabidopsis cis-prenyltransferase itself could not catalyze the formation of high molecular weight polyprenyl diphosphate such as natural rubber | Arabidopsis thaliana | 21 diphosphate + di-trans,poly-cis tetracosaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 21 isopentenyl diphosphate | trans,trans-farnesyl diphosphate is a better substrate than geranylgeranyl diphosphate | Arabidopsis thaliana | 21 diphosphate + di-trans,poly-cis tetracosaprenyl diphosphate | the enzyme synthesizes polyisoprenes with carbon number higher than 90. The peak activity is observed at the point that corresponds to the polyisoprene with carbon number C120 | ? | |
| geranylgeranyl diphosphate + 20 isopentenyl diphosphate | trans,trans-farnesyl diphosphate is a better substrate than geranylgeranyl diphosphate | Arabidopsis thaliana | 20 diphosphate + di-trans,poly-cis-tetracosaprenyl diphosphate | the enzyme synthesizes polyisoprenes with carbon number higher than 90. The peak activity is observed at the point that corresponds to the polyisoprene with carbon number C120 | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, calculated from sequence | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AcpT | - |
Arabidopsis thaliana |
| dehydrodolichyl diphosphate synthase 1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | calculated from sequence | - |
8.05 |
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