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Literature summary for 2.5.1.86 extracted from

  • Kaur, D.; Brennan, P.J.; Crick, D.C.
    Decaprenyl diphosphate synthesis in Mycobacterium tuberculosis (2004), J. Bacteriol., 186, 7564-7570.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.86

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol stimulates activity Mycobacterium tuberculosis
Triton X-100 detergent stimulates activity, maximally active in presence of 0.1% Triton X-100 Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 neryl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.04
-
 geranylgeranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.084
-
 (2E,6E)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.089
-
 isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.29
-
 (2Z,6E)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.49
-
 geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective Mycobacterium tuberculosis
Mg2+ absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective Mycobacterium tuberculosis
Mn2+ absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective Mycobacterium tuberculosis
Zn2+ absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Mycobacterium tuberculosis the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate
-
 ?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Mycobacterium tuberculosis H37Rv the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WFF7
-
-
Mycobacterium tuberculosis H37Rv P9WFF7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate the relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate. Shorter chain length also produced. As the concentration of (2Z,6E)-farnesyl diphosphate is increased in the assays, there is an increase in the amount of the shorter products synthesized relative to decaprenyl diphosphate ?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis H37Rv diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate the relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate. Shorter chain length also produced. As the concentration of (2Z,6E)-farnesyl diphosphate is increased in the assays, there is an increase in the amount of the shorter products synthesized relative to decaprenyl diphosphate ?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo Mycobacterium tuberculosis 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate
-
 ?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate (2Z,6E)-farnesyl diphosphate i.e. omega, E,Z-farnesyl diphosphate, the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor Mycobacterium tuberculosis 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate decaprenyl diphosphate is the predominant product synthesized, smaller amounts of intermediates of intermediates of shorter chain lengths also being produced. The amount of 40-carbon and 45-carbon products relative to 50-carbon products increases as the (2Z,6E)-farnesyl diphosphate concentration is increased, suggesting that the enzyme loses specificity as the substrate concentration increases ?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo Mycobacterium tuberculosis H37Rv 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate
-
 ?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate (2Z,6E)-farnesyl diphosphate i.e. omega, E,Z-farnesyl diphosphate, the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor Mycobacterium tuberculosis H37Rv 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate decaprenyl diphosphate is the predominant product synthesized, smaller amounts of intermediates of intermediates of shorter chain lengths also being produced. The amount of 40-carbon and 45-carbon products relative to 50-carbon products increases as the (2Z,6E)-farnesyl diphosphate concentration is increased, suggesting that the enzyme loses specificity as the substrate concentration increases ?
geranyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate decaprenyl diphosphate, nonaprenyl diphosphate, and octaprenyl diphosphate are synthesized. The relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate ?
geranyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis H37Rv diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate decaprenyl diphosphate, nonaprenyl diphosphate, and octaprenyl diphosphate are synthesized. The relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate ?
geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis diphosphate + decaprenyl diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate the major product synthesized is octaprenyl diphosphate, followed by nonaprenyl diphosphate, decaprenyl diphosphate, and heptaprenyl diphosphate ?
additional information the enzyme is not strictly specific for either the chain length or stereoconfiguration of the acceptor. No activity with dimethylallyl diphosphate Mycobacterium tuberculosis ?
-
 ?
additional information the enzyme is not strictly specific for either the chain length or stereoconfiguration of the acceptor. No activity with dimethylallyl diphosphate Mycobacterium tuberculosis H37Rv ?
-
 ?
neryl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate decaprenyl diphosphate, nonaprenyl diphosphate, and octaprenyl diphosphate are synthesized. The relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
Rv2361c
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 geranylgeranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.06
-
 geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.07
-
 (2E,6E)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.08
-
 isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.16
-
 neryl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
11.5
-
 (2Z,6E)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.122
-
 geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.75
-
 geranylgeranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.899
-
 isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
5.5
-
 neryl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
39.66
-
 (2Z,6E)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis