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Literature summary for 2.5.1.85 extracted from
- Cloning and kinetic characterization of Arabidopsis thaliana solanesyl diphosphate synthase (2003), Biochem. J., 370, 679-686.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously expressed in Escherichia coli as as His6-tagged fusion protein | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.61 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana |  |
| 5.73 | - |
(2E,6E)-farnesyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
| 20 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate | Arabidopsis thaliana | |
| 151 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46600 | - |
2 * 46600, His6-tagged protein, calculated from sequence | Arabidopsis thaliana |
| 108000 | - |
gel filtration | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8S948 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | geranylgeranyl diphosphate is preferred over farnesyl diphosphate as allylic substrate | Arabidopsis thaliana | 6 diphosphate + all-trans-nonaprenyl diphosphate | the main product is nonaprenyl diphosphate, considerable amounts of geranylgeranyl diphosphate are formed | ? | |
| geranylgeranyl diphosphate + 5 isopentenyl diphosphate | geranylgeranyl diphosphate is preferred over farnesyl diphosphate as allylic substrate | Arabidopsis thaliana | 5 diphosphate + all-trans-nonaprenyl diphosphate | nonaprenyl diphosphate is almost exclusively formed | ? | |
| additional information | no activity with farnesyl diphosphate and dimethylallyl diphosphate | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46600, His6-tagged protein, calculated from sequence | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| At-SPS1 | - |
Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 8.0, 10 minm, stable below | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.7 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate | Arabidopsis thaliana | |
| 15 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate | Arabidopsis thaliana | |
| 21 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
| 29.7 | - |
farnesyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0968 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate | Arabidopsis thaliana | |
| 0.752 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate | Arabidopsis thaliana | |
| 5.19 | - |
(2E,6E)-farnesyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
| 13.1 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
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