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Literature summary for 2.5.1.84 extracted from

  • Ohara, K.; Sasaki, K.; Yazaki, K.
    Two solanesyl diphosphate synthases with different subcellular localizations and their respective physiological roles in Oryza sativa (2010), J. Exp. Bot., 61, 2683-2692.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in yeast Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
Oryza sativa 9536
-
plastid isoform SPS2 shows a putative transit peptide of 57 amino acids for plastidal localization at the N-terminus Oryza sativa 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
farnesyl diphosphate + 6 isopentenyl diphosphate Oryza sativa OsSP2 is involved in the supply of all-trans-nonaprenyl diphosphate for plastoquinone-9 biosynthesis 6 diphosphate + nonaprenyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-
Oryza sativa Q75HZ9 isoform SPS2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification isoform SPS2 shows a putative transit peptide of 57 amino acids for plastidal localization at the N-terminus Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf high expression Oryza sativa
-
leaf high expression level Oryza sativa
-
root high expression Oryza sativa
-
root high expression level Oryza sativa
-
stem low expression Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate 44% of the rate with geranyl diphosphate Oryza sativa 6 diphosphate + all-trans-nonaprenyl diphosphate products range from C 15 to C45 ?
dimethylallyl diphosphate + 8 isopentenyl diphosphate 18.1% of the activity with geranyl diphosphate, OsSPS2 Oryza sativa 8 diphosphate + nonaprenyl diphosphate
-
?
dimethylallyl diphosphate + isopentenyl diphosphate 18% of the rate with geranyl diphosphate Oryza sativa diphosphate + ?
-
?
farnesyl diphosphate + 6 isopentenyl diphosphate OsSP2 is involved in the supply of all-trans-nonaprenyl diphosphate for plastoquinone-9 biosynthesis Oryza sativa 6 diphosphate + nonaprenyl diphosphate
-
?
farnesyl diphosphate + 6 isopentenyl diphosphate 44% of the activity with geranyl diphosphate, OsSPS2 Oryza sativa 6 diphosphate + nonaprenyl diphosphate
-
?
geranyl diphosphate + 7 isopentenyl diphosphate best substrate Oryza sativa 7 diphosphate + all-trans-nonaprenyl diphosphate
-
?
geranyl diphosphate + 7 isopentenyl diphosphate geranyl diphosphate is the preferred substrate, OsSPS2 Oryza sativa 7 diphosphate + nonaprenyl diphosphate
-
?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate 35% of the rate with farnesyl diphosphate Oryza sativa 5 diphosphate + all-trans-nonaprenyl diphosphate products range from C 15 to C45 ?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate 34.6% of the activity with geranyl diphosphate, OsSPS2 Oryza sativa 5 diphosphate + nonaprenyl diphosphate
-
?

Synonyms

Synonyms Comment Organism
OsSPS2
-
Oryza sativa
Sps2
-
Oryza sativa

General Information

General Information Comment Organism
physiological function isoform SPS2 weakly complements the yeast coq1 disruptant, which does not form ubiquinone. Transformants produce ubiquinone 9 instead of the native yeast product ubiquinone 6. SPS2 is involved in providing solanesyl diphosphate for plastochinone-9 formation Oryza sativa