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Literature summary for 2.5.1.81 extracted from
- Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution (2010), Biochem. Biophys. Res. Commun., 393, 16-20.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Methanosarcina mazei |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activity around 5.0 mM, no activity with other divalent cations such as Ca2+, Mn2+, and Zn2+ | Methanosarcina mazei |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE, calculated from sequence | Methanosarcina mazei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| farnesyl diphosphate + 2 isopentenyl diphosphate | Methanosarcina mazei | biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + 2 diphosphate | - |
? | |
| geranylgeranyl diphosphate + isopentenyl diphosphate | Methanosarcina mazei | biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina mazei | Q8PYS1 | Gö1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina mazei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| farnesyl diphosphate + 2 isopentenyl diphosphate | biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis | Methanosarcina mazei | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + 2 diphosphate | - |
? | |
| farnesyl diphosphate + 2 isopentenyl diphosphate | the enzyme prefers geranylgeranyl diphosphate and farnesyl diphosphate as allylic substrate | Methanosarcina mazei | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + 2 diphosphate | (2E,6E,10E,14E)-geranylfarnesyl diphosphate is the main product, a considerable amount of geranylgeranyl diphosphate as an intermediate is also observed | ? | |
| geranyl diphosphate + 3 isopentenyl diphosphate | slight activity | Methanosarcina mazei | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + 3 diphosphate | - |
? | |
| geranylgeranyl diphosphate + isopentenyl diphosphate | biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis | Methanosarcina mazei | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? | |
| geranylgeranyl diphosphate + isopentenyl diphosphate | the enzyme prefers geranylgeranyl diphosphate and farnesyl diphosphate as allylic substrate | Methanosarcina mazei | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? | |
| additional information | dimethylally diphosphate is not accepted as substrate | Methanosarcina mazei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE, calculated from sequence | Methanosarcina mazei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MM_0789 | - |
Methanosarcina mazei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Methanosarcina mazei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Methanosarcina mazei |
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