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Literature summary for 2.5.1.78 extracted from

  • Min, J.; Kim, S.; Lee, J.; Kang, S.
    Lumazine synthase protein cage nanoparticles as modular delivery platforms for targeted drug delivery (2014), RSC Adv., 4, 48596-48600.
No PubMed abstract available
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Application

Application Comment Organism
molecular biology outside of the cell, the hollow spherical architecture of the enzyme capsid is used as a template for the encapsulation of cargo proteins, such as green fluorescent proteins, and HIV proteases, and fabrication of uniform layer-by-layer assemblies using non-covalent interactions between surface-displayed His6 and Ni-NTA of enzyme AaLS. The enzyme shows encapsulation capability and surface presentation of ligands, which represent the great potential of AaLS as a versatile delivery vehicle Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
additional information development of lumazine synthase, isolated from hyperthermophile Aquifex aeolicus, as a modular delivery nanoplatform for the targeted delivery of diagnostic and/or therapeutic molecules depending on target cancer cells, method evaluation, overview. The enzyme is engineered for its binding and transport/ligand presentation function by mutation of rg108 to Cys, and insertion the RGD4C peptide with extra linker sequences (GGGCDCRGDCFCASGGG) to the position between residues 70 and 71, where the exterior loop is formed, allowing RGD4C peptides to adopt an active cyclic form through intramolecular disulfide bonds. In parallel, the SP94 peptide (SFSIIHTPILPL) is introduced at the C-terminal end of AaLS because the C-termini are on the exterior surface based on atomic resolution structural information and the linear form of SP94 peptide being the most active, ESI-TOF MS, UV/Vis spectrophotometric, and size-exclusion chromatographic analysis, overview. The construct binds selectively to their target cells, KB or HepG2 cells. Conjugation of bifunctional N-(3,4-dihydroxyphenethyl)-3-maleimido-propanamide to SP94-AaLS via a thiolmaleimide Michael-type addition and introduction of anti-cancer drug bortezomib (BTZ). The cytoxicity of HepG2 cells treated with BTZ-Catechol-SP94-AaLS also increases in a dose-dependent manner, similar to that of free BTZ, while SP94-AaLS without BTZ has almost no cytotoxicity on the cells Aquifex aeolicus
R108C site-directed mutagenesis Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
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Subunits

Subunits Comment Organism
More enzyme AaLS consists of 60 identical subunits that form an icosahedral capsid architecture with 15.4 nm exterior and 9 nm interior diameters Aquifex aeolicus

Synonyms

Synonyms Comment Organism
AaLS
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 Aquifex aeolicus
lumazine synthase
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 Aquifex aeolicus

General Information

General Information Comment Organism
metabolism enzyme AaLS catalyzes the penultimate step in riboflavin biosynthesis Aquifex aeolicus