Application | Comment | Organism |
---|---|---|
medicine | partially protective effect of enzyme BLS against B16 melanoma in mice generating a protective response at the first stages of tumor growth but not at later stages, therapeutic effect of BLS vaccination | Brucella sp. |
Cloned (Comment) | Organism |
---|---|
recombinant expression of enzyme BLS in Escherichia coli strain BL21 (DE3) | Brucella sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | for evaluation of the effectiveness of enzyme BLS as a preventive vaccine, C57BL/6J mice are immunized with BLS or BLS-OVA, subcutaneous inoculation with B16-OVA melanoma, and analysis of the effect induced by BLS in TLR4-expressing B16 melanoma cells. BLS or BLS-OVA induce a significant inhibition of tumor growth, survival is increased, and 50% of mice immunized with the highest dose of BLS do not develop visible tumors | Brucella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella sp. | P61711 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme BLS from Escherichia coli strain BL21 (DE3) by dialysis, anion exchange chromatography, and gel filtration | Brucella sp. |
Subunits | Comment | Organism |
---|---|---|
decamer | - |
Brucella sp. |
Synonyms | Comment | Organism |
---|---|---|
BLS | - |
Brucella sp. |
lumazine synthase | - |
Brucella sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | lumazine synthase (BLS) is a highly immunogenic decameric protein which can accept the fusion of foreign proteins at its ten N-termini. These chimeras are very efficient to elicit systemic and oral immunity without adjuvants. BLS signaling via Toll-like receptor 4 (TLR4) regulates innate and adaptive immune responses, inducing dendritic cell maturation and CD8+ T-cell cytotoxicity | Brucella sp. |