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Literature summary for 2.5.1.78 extracted from

  • Mejias, M.P.; Ghersi, G.; Craig, P.O.; Panek, C.A.; Bentancor, L.V.; Baschkier, A.; Goldbaum, F.A.; Zylberman, V.; Palermo, M.S.
    Immunization with a chimera consisting of the B subunit of Shiga toxin type 2 and Brucella lumazine synthase confers total protection against Shiga toxins in mice (2013), J. Immunol., 191, 2403-2411.
    View publication on PubMed
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Application

Application Comment Organism
medicine the striking feature of enterohemorrhagic Escherichia coli (EHEC) infections is the production of Shiga toxins (Stx) implicated in the development of the life-threatening hemolytic uremic syndrome. Immunization with a chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase confers total protection against Shiga toxins in mice, analysis of antibody efficacy against EHEC challenge. The enzyme can be used as transport vehhicle and antigen in vaccination against EHEC infections Brucella sp.

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the enzyme in Escherichia coli, and of the enzyme fused to Shiga toxin 2 subunit B, resulting in the BLS-Stx2B chimera, in Escherichia coli strain JM109 Brucella sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase, BLS-Stx2B chimera, confers total protection against Shiga toxins in mice after immunization Brucella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
234000
-
 recombinant chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase, i.e. BLS-Stx2B chimera, gel filtration Brucella sp.

Organism

Organism UniProt Comment Textmining
Brucella sp.
-
-
-

Subunits

Subunits Comment Organism
More BLS folds as a decamer in the recombinant BLS-Stx2B chimera, i.e. a chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase Brucella sp.

Synonyms

Synonyms Comment Organism
BLS
-
 Brucella sp.
lumazine synthase
-
 Brucella sp.

General Information

General Information Comment Organism
additional information molecular modelling of the recombinant BLS-Stx2B chimera fusing the C-terminal end of the structure of Stx2B, PDB ID IR4P, with the N-terminus of the crystallographic structure of enzyme BLS, PDB ID IDI0, through a decapeptide linker of sequence GSGSGSGSGS Brucella sp.