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Literature summary for 2.5.1.78 extracted from

  • Alfano, E.; Lentz, E.; Bellido, D.; Dus Santos, M.; Goldbaum, F.; Wigdorovitz, A.; Bravo-Almonacid, F.
    Expression of the multimeric and highly immunogenic Brucella spp. lumazine synthase fused to bovine rotavirus VP8D as a scaffold for antigen production in tobacco chloroplasts (2015), Front. Plant Sci., 6, 1170.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
medicine the BLS scaffold is assessed for in plants for recombinant vaccine development by N-terminally fusing BLS to bovine rotavirus VP8d and expressing the resulting fusion (BLS-VP8d) in Nicotiana tabacum chloroplasts. BLS-VP8d remains soluble and stable during all stages of plant development and even in lyophilized leaves stored at room temperature Brucella sp.

Cloned(Commentary)

Cloned (Comment) Organism
construction of a fusion protein with enzyme BLS and inner core domain (VP8d) of VP8 spike protein from bovine rotavirus, subcloning in Escherichia coli, expression of the BLS-VP8d fusion protein in Nicotiana tabacum cv. Petit Havana leaf chloroplasts to evaluate the possible use of this antigen for vaccine development Brucella sp.

Protein Variants

Protein Variants Comment Organism
additional information chloroplast transformation vector and analysis of transgene integration. The BLS scaffold is assessed for in plants for recombinant vaccine development by N-terminally fusing BLS to bovine rotavirus VP8d and expressing the resulting fusion (BLS-VP8d) in Nicotiana tabacum chloroplasts. BLS-VP8d remains soluble and stable during all stages of plant development and even in lyophilized leaves stored at room temperature Brucella sp.

Organism

Organism UniProt Comment Textmining
Brucella sp. P61711
-
-
Brucella sp. 9-941 P61711
-
-

Subunits

Subunits Comment Organism
decamer
-
 Brucella sp.

Synonyms

Synonyms Comment Organism
BLS
-
 Brucella sp.
lumazine synthase
-
 Brucella sp.

General Information

General Information Comment Organism
physiological function lumazine synthase from Brucella spp.is a highly immunogenic decameric protein which can accommodate foreign polypeptides or protein domains fused to its N-termini, markedly increasing their immunogenicity Brucella sp.