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Literature summary for 2.5.1.78 extracted from

  • Birkenmeier, M.; Neumann, S.; Roeder, T.
    Kinetic modeling of riboflavin biosynthesis in Bacillus subtilis under production conditions (2014), Biotechnol. Lett., 36, 919-928.
    View publication on PubMed
Search for more literature for 2.5.1.78

Application

Application Comment Organism
synthesis Bacillus subtilis is used for riboflavin production, also involving the enzyme RibH Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics, and kinetic modelling of the interaction of the single enzymes and identification of rate-limiting steps in the biosynthetic pathway, detailed overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione + 3,4-dihydroxy-2-butanone 4-phosphate Bacillus subtilis
-
 6,7-dimethyl-8-ribityllumazine + 2 H2O + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P11998 gene ribH
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione + 3,4-dihydroxy-2-butanone 4-phosphate
-
 Bacillus subtilis 6,7-dimethyl-8-ribityllumazine + 2 H2O + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
lumazine synthase
-
 Bacillus subtilis
RibH
-
 Bacillus subtilis

General Information

General Information Comment Organism
metabolism the enzyme is involved in the riboflavin biosynthesis pathway. The RibA protein is rate limiting in the pathway, reductase activity of RibG and lumazine synthase (RibH) might be the next most limiting steps. Computational minimization of the enzyme concentrations of the pathway suggests the need for a greater RibH enzyme concentration (0.251 mM) compared with the other enzymes (RibG 0.188 mM, RibB 0.023 mM). Kinetic modelling of the interaction of the single enzymes and identification of rate-limiting steps in the biosynthetic pathway. The potential order of enzyme limitation under increasing RibA concentration is as follows: the reductase activity of RibG and the lumazine synthase (RibH) are rather more limiting than riboflavin synthase (RibB) and the deaminase activity of RibG Bacillus subtilis
physiological function the enzyme is involved in the riboflavin biosynthesis pathway. Riboflavin or vitamin B2 is the precursor of the flavin cofactors flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), and is used commercially as an animal feed supplement and as a food colorant (E101) Bacillus subtilis