Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
A56S | kcat is 81.3% of wild-type value | Bacillus subtilis |
D138A | kcat is 91.5% of wild-type value | Bacillus subtilis |
E58Q | kcat is 70.2% of wild-type value | Bacillus subtilis |
F113S | kcat is 6.8% of wild-type value | Bacillus subtilis |
F22D | kcat is 14.5% of wild-type value | Bacillus subtilis |
F22S | kcat is 47.2% of wild-type value | Bacillus subtilis |
F22V | kcat is 26.4% of wild-type value | Bacillus subtilis |
F22W | kcat is 43.8% of wild-type value | Bacillus subtilis |
F57S | kcat is 43.8% of wild-type value | Bacillus subtilis |
H88A | kcat is 12% of wild-type value | Bacillus subtilis |
H88K | kcat is 39.5% of wild-type value | Bacillus subtilis |
K131N | kcat is 9.7% of wild-type value | Bacillus subtilis |
K131R | kcat is 29.8% of wild-type value | Bacillus subtilis |
K135A | kcat is 21.9% of wild-type value | Bacillus subtilis |
N23S | kcat is 21.9% of wild-type value | Bacillus subtilis |
R127H | kcat is 69.7% of wild-type value | Bacillus subtilis |
S142L | kcat is 62.3% of wild-type value | Bacillus subtilis |
T80V | kcat is 55.1% of wild-type value | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme T80V | Bacillus subtilis | |
0.0042 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme A56S | Bacillus subtilis | |
0.00612 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F22W | Bacillus subtilis | |
0.00857 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, wild-type enzyme | Bacillus subtilis | |
0.01 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme D138A | Bacillus subtilis | |
0.0107 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F22V | Bacillus subtilis | |
0.011 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme S142L | Bacillus subtilis | |
0.0115 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme K135A | Bacillus subtilis | |
0.0119 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme N23S | Bacillus subtilis | |
0.0138 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme K131R | Bacillus subtilis | |
0.0345 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F57S | Bacillus subtilis | |
0.0355 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme K131N | Bacillus subtilis | |
0.042 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme H88K | Bacillus subtilis | |
0.042 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme T80V | Bacillus subtilis | |
0.05 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme K131R | Bacillus subtilis | |
0.0546 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, wild-type enzyme | Bacillus subtilis | |
0.056 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme H88K | Bacillus subtilis | |
0.0581 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme N23S | Bacillus subtilis | |
0.0598 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F22W | Bacillus subtilis | |
0.06 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme H88A | Bacillus subtilis | |
0.0636 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme D138A | Bacillus subtilis | |
0.0665 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F113S | Bacillus subtilis | |
0.0702 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme A56S | Bacillus subtilis | |
0.08 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F57S | Bacillus subtilis | |
0.0849 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme R127H | Bacillus subtilis | |
0.0905 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme S142L | Bacillus subtilis | |
0.124 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F22V | Bacillus subtilis | |
0.137 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F22S | Bacillus subtilis | |
0.14 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F22S | Bacillus subtilis | |
0.146 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme E58Q | Bacillus subtilis | |
0.147 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme H88A | Bacillus subtilis | |
0.167 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme K135A | Bacillus subtilis | |
0.173 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme E58Q | Bacillus subtilis | |
0.278 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F113S | Bacillus subtilis | |
0.283 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme K131N | Bacillus subtilis | |
0.675 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F22D | Bacillus subtilis | |
0.72 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F22D | Bacillus subtilis | |
3.14 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme R127H | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (3R)-3,4-dihydroxy-2-butanone 4-phosphate | - |
Bacillus subtilis | 6,7-dimethyl-8-(1-D-ribityl)lumazine + 2 H2O + phosphate | - |
? | |
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | the rate enhancement by the enzyme is predominantly achieved by establishing a favourable topological relation of the two substrates, whereas acid/base catalysis may play a secondary role | Bacillus subtilis | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RibH | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme H88K | Bacillus subtilis | |
0.0022 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme H88K | Bacillus subtilis | |
0.0038 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F113S | Bacillus subtilis | |
0.0038 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F113S | Bacillus subtilis | |
0.0054 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme K131N | Bacillus subtilis | |
0.0054 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme K131N | Bacillus subtilis | |
0.0067 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme H88A | Bacillus subtilis | |
0.0067 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme H88A | Bacillus subtilis | |
0.0081 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F22D | Bacillus subtilis | |
0.0081 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F22D | Bacillus subtilis | |
0.0122 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme K135A | Bacillus subtilis | |
0.0122 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme K135A | Bacillus subtilis | |
0.0122 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme N23S | Bacillus subtilis | |
0.0122 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme N23S | Bacillus subtilis | |
0.0147 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F22V | Bacillus subtilis | |
0.0147 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F22V | Bacillus subtilis | |
0.0166 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme K131R | Bacillus subtilis | |
0.0166 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme K131R | Bacillus subtilis | |
0.0244 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F22W | Bacillus subtilis | |
0.0244 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F22W | Bacillus subtilis | |
0.0244 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F57S | Bacillus subtilis | |
0.0244 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F57S | Bacillus subtilis | |
0.0263 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme F22S | Bacillus subtilis | |
0.0263 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme F22S | Bacillus subtilis | |
0.0307 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme T80V | Bacillus subtilis | |
0.0307 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme T80V | Bacillus subtilis | |
0.0347 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme S142L | Bacillus subtilis | |
0.0347 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme S142L | Bacillus subtilis | |
0.0389 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme R127H | Bacillus subtilis | |
0.0389 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme R127H | Bacillus subtilis | |
0.0391 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme E58Q | Bacillus subtilis | |
0.0391 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme E58Q | Bacillus subtilis | |
0.0453 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme A56S | Bacillus subtilis | |
0.0453 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme A56S | Bacillus subtilis | |
0.051 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme D138A | Bacillus subtilis | |
0.051 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme D138A | Bacillus subtilis | |
0.0557 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, wild-type enzyme | Bacillus subtilis | |
0.0557 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, wild-type enzyme | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus subtilis |