Literature summary for 2.5.1.78 extracted from

Meining, W.; M�rtl, S.; Fischer, M.; Cushman, M.; Bacher, A.; Ladenstein, R.
The atomic structure of pentameric lumazine synthase from Saccharomyces cerevisiae at 1.85 A resolution reveals the binding mode of a phosphonate intermediate analogue (2000), J. Mol. Biol., 299, 181-197.

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting-drop vapour-diffusion method. Crystals of the recombinant enzyme with a size of up to 1.6 mm are obtained. The space group is P4(1)2(1)2 with lattice dimensions 82.9 A x 82.9 A x 300.2 A. X-ray diffraction data collected under cryogenic conditions are complete to 1.85 A resolution. The structure of the enzyme in complex with the intermediate analogue, 5-(6-D-ribitylamino-2,4-dihydroxypyrimidine-5-yl)-1-pentyl-phosphonic acid is solved via molecular replacement using the structure of the Bacillus subtilis enzyme as search model and is refined to a final R-factor of 19.8%	Saccharomyces cerevisiae

Crystallization (Comment)

Organism

sitting-drop vapour-diffusion method. Crystals of the recombinant enzyme with a size of up to 1.6 mm are obtained. The space group is P4(1)2(1)2 with lattice dimensions 82.9 A x 82.9 A x 300.2 A. X-ray diffraction data collected under cryogenic conditions are complete to 1.85 A resolution. The structure of the enzyme in complex with the intermediate analogue, 5-(6-D-ribitylamino-2,4-dihydroxypyrimidine-5-yl)-1-pentyl-phosphonic acid is solved via molecular replacement using the structure of the Bacillus subtilis enzyme as search model and is refined to a final R-factor of 19.8%

Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Subunits

Subunits	Comment	Organism
pentamer	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)