Crystallization (Comment) | Organism |
---|---|
crystal structure analysis of reconstituted, icosahedral beta-subunit capsids with bound substrate analogue inhibitor (5-nitro-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione) at 2.4 A resolution | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-nitro-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione | - |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
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Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | penultimate step of riboflavin biosynthesis | Bacillus subtilis | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Subunits | Comment | Organism |
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More | the bifunctional lumazine synthase/riboflavin synthase (EC 2.5.1.9) complex is composed of 3 alpha (riboflavin synthase) subunits and 60 beta (lumazine synthase) subunits and has a relative mass of 1 MDa. The 60 beta subunits are arranged in an icosahedral capsid enclosing the alpha trimer in the central core. Hollow, icosahedral capsids consisting of 60 beta subunits can be obtained by inhibitor-driven renaturation of isolated beta subunits. They catalyse the formation of 6,7-dimethyl-8-ribityllumazine at the same rate as the native alpha3beta60 complex and can be crystallised in two different hexagonal and one monoclinic form | Bacillus subtilis |
Synonyms | Comment | Organism |
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lumazine synthase/riboflavin synthase complex | bifunctional enzyme complex | Bacillus subtilis |