Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.78 extracted from

  • Garcia-Ramirez, J.J.; Santos, M.A.; Revuelta, J.L.
    The Saccharomyces cerevisiae RIB4 gene codes for 6,7-dimethyl-8-ribityllumazine synthase involved in riboflavin biosynthesis. Molecular characterization of the gene and purification of the encoded protein (1995), J. Biol. Chem., 270, 23801-23807.
    View publication on PubMed
Search for more literature for 2.5.1.78

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18600
-
 5 * 18600, calculated from sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Saccharomyces cerevisiae the enzyme is involved in biosynthesis of riboflavin. Gene disruption of the chromosomal copy of RIB4 leads to riboflavin auxotrophy and loss of enzyme activity 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P50861
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpression of the RIB4 coding sequence in yeast cells under the control of the strong TEF1 promoter allowed ready purification of 6,7-dimethyl-8-ribityllumazine synthase to apparent homogeneity by a simple procedure Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Saccharomyces cerevisiae 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate the enzyme is involved in biosynthesis of riboflavin. Gene disruption of the chromosomal copy of RIB4 leads to riboflavin auxotrophy and loss of enzyme activity Saccharomyces cerevisiae 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
pentamer 5 * 18600, calculated from sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
6,7-dimethyl-8-ribityllumazine synthase
-
 Saccharomyces cerevisiae
RIB4
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae