Literature summary for 2.5.1.78 extracted from

Rodriguez-Fernandez, L.; Lopez-Jaramillo, F.J.; Bacher, A.; Fischer, M.; Weinkauf, S.
Improvement of the quality of lumazine synthase crystals by protein engineering (2008), Acta Crystallogr. Sect. F, 64, 625-628.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
native protein, 2.4 A resolution, space group P6322 or C2. Mutant D44G/C93S/C139S/T118A crystallizes in space group R3 and diffracts to 1.6 A resolution	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
D44G/C93S/C139S/T118A	mutant constructed to improve the overexpression and purification of the molecule as well as to obtain new crystal forms. Two cysteines are replaced to bypass misfolding problems and a charged surface residue is replaced to force different molecular packings. Mutant crystallizes in space group R3 and diffracts to 1.6 A resolution	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P11998	-	-

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Release 2023.1 (January 2023)