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Literature summary for 2.5.1.78 extracted from

  • Kis, K.; Bacher, A.
    Substrate channeling in the lumazine synthase/riboflavin synthase complex of Bacillus subtilis (1995), J. Biol. Chem., 270, 16788-16795.
    View publication on PubMed
Search for more literature for 2.5.1.78

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione native enzyme complex, pH 7.0, 37°C Bacillus subtilis
0.13
-
 (3S)-3,4-dihydroxy-2-butanone native enzyme complex, pH 7.0, 37°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (3R)-3,4-dihydroxy-2-butanone 4-phosphate
-
 Bacillus subtilis 6,7-dimethyl-8-(1-D-ribityl)lumazine + 2 H2O + phosphate
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (3S)-3,4-dihydroxy-2-butanone
-
 Bacillus subtilis ?
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate lumazine synthase/riboflavin synthase complex, the beta subunit carries lumazine synthase activity. Product channeling for subsequent synthesis of riboflavin by riboflavin synthase present in the complex Bacillus subtilis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione
-
 Bacillus subtilis ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.056
-
 L-3,4-dihydroxybutan-2-one 4-phosphate native enzyme complex, pH 7.0, 37°C Bacillus subtilis
0.056
-
 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione native enzyme complex, pH 7.0, 37°C Bacillus subtilis
0.076
-
 L-3,4-dihydroxybutan-2-one 4-phosphate hollow beta60 capsid, pH 7.0, 37°C Bacillus subtilis
0.076
-
 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione hollow beta60 capsid, pH 7.0, 37°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bacillus subtilis