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Literature summary for 2.5.1.75 extracted from

  • Subedi, B.P.; Corder, A.L.; Zhang, S.; Foss, F.W.; Pierce, B.S.
    Steady-state kinetics and spectroscopic characterization of enzyme-tRNA interactions for the non-heme diiron tRNA-monooxygenase, MiaE (2015), Biochemistry, 54, 363-376.
    View publication on PubMed
Search for more literature for 2.5.1.75

Cloned(Commentary)

Cloned (Comment) Organism
gene miaA, DNA and amino acid sequence determination and analysis, recombinant expression of the enzyme as maltose-binding-protein fusion protein in Escherichia coli strain BL21(DE3) Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten steady-state kinetics Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + adenine37 in tRNA Salmonella enterica
-
 diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
dimethylallyl diphosphate + adenine37 in tRNA Salmonella enterica ATCC 700720
-
 diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica P37724 gene miaA
-
Salmonella enterica ATCC 700720 P37724 gene miaA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, tag cleavage by TEV protease, and Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + adenine37 in tRNA
-
 Salmonella enterica diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
dimethylallyl diphosphate + adenine37 in tRNA
-
 Salmonella enterica ATCC 700720 diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
dimethylallyl diphosphate + adenine37 in tRNATrp isopentenylaion of the A37 within the ACSLTrp sequence, detailed spectrometrical reaction analysis, overview Salmonella enterica diphosphate + N6-dimethylallyladenine37 in tRNATrp
-
 ?
dimethylallyl diphosphate + adenine37 in tRNATrp isopentenylaion of the A37 within the ACSLTrp sequence, detailed spectrometrical reaction analysis, overview Salmonella enterica ATCC 700720 diphosphate + N6-dimethylallyladenine37 in tRNATrp
-
 ?
additional information the minimal substrate for bacterial MiaA is the anticodon stem loop (ACSL) of targeted tRNA molecules Salmonella enterica ?
-
 ?
additional information the minimal substrate for bacterial MiaA is the anticodon stem loop (ACSL) of targeted tRNA molecules Salmonella enterica ATCC 700720 ?
-
 ?

Synonyms

Synonyms Comment Organism
dimethylallyl (DELTA2-isopentenyl)diphosphate-tRNA transferase
-
 Salmonella enterica
MiaA
-
 Salmonella enterica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Salmonella enterica

General Information

General Information Comment Organism
evolution while absent in eukaryotes, some facultative bacteria use a non-heme diiron enzyme [MiaE, 2-methylthio-N6-isopentenyladenosine(37)-tRNA monooxygenase] to catalyze the O2-dependent hydroxylation of ms2i6A37 to produce 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37), comparison of MiaA and MiaE catalytic reactions, overview Salmonella enterica
metabolism the enzyme is involved in the hypermodification enzyme pathway for 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37) synthesis. within the anticodon stem loop of tRNA, nucleotide modifications are frequently observed at position 34 (the wobble position) or position 37 (3' adjacent to the anticodon), nearly all eukaryotic and bacterial tRNAs that read codons starting with U are modified by the enzymes MiaA and MiaB and contain the ms2i6A modification Salmonella enterica
physiological function enzyme MiaA catalyzes the nucleophilic substitution of the dimethylallyl group from dimethylallyl diphosphate (DMAPP) to the exocyclic 6-amino nitrogen of A37 (N6) to yield N6-isopentenyladenosine (i6A37) with release of inorganic diphosphate Salmonella enterica