Cloned (Comment) | Organism |
---|---|
gene miaA, DNA and amino acid sequence determination and analysis, recombinant expression of the enzyme as maltose-binding-protein fusion protein in Escherichia coli strain BL21(DE3) | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + adenine37 in tRNA | Salmonella enterica | - |
diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
dimethylallyl diphosphate + adenine37 in tRNA | Salmonella enterica ATCC 700720 | - |
diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | P37724 | gene miaA | - |
Salmonella enterica ATCC 700720 | P37724 | gene miaA | - |
Purification (Comment) | Organism |
---|---|
recombinant MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, tag cleavage by TEV protease, and | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + adenine37 in tRNA | - |
Salmonella enterica | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
dimethylallyl diphosphate + adenine37 in tRNA | - |
Salmonella enterica ATCC 700720 | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
dimethylallyl diphosphate + adenine37 in tRNATrp | isopentenylaion of the A37 within the ACSLTrp sequence, detailed spectrometrical reaction analysis, overview | Salmonella enterica | diphosphate + N6-dimethylallyladenine37 in tRNATrp | - |
? | |
dimethylallyl diphosphate + adenine37 in tRNATrp | isopentenylaion of the A37 within the ACSLTrp sequence, detailed spectrometrical reaction analysis, overview | Salmonella enterica ATCC 700720 | diphosphate + N6-dimethylallyladenine37 in tRNATrp | - |
? | |
additional information | the minimal substrate for bacterial MiaA is the anticodon stem loop (ACSL) of targeted tRNA molecules | Salmonella enterica | ? | - |
? | |
additional information | the minimal substrate for bacterial MiaA is the anticodon stem loop (ACSL) of targeted tRNA molecules | Salmonella enterica ATCC 700720 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dimethylallyl (DELTA2-isopentenyl)diphosphate-tRNA transferase | - |
Salmonella enterica |
MiaA | - |
Salmonella enterica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Salmonella enterica |
General Information | Comment | Organism |
---|---|---|
evolution | while absent in eukaryotes, some facultative bacteria use a non-heme diiron enzyme [MiaE, 2-methylthio-N6-isopentenyladenosine(37)-tRNA monooxygenase] to catalyze the O2-dependent hydroxylation of ms2i6A37 to produce 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37), comparison of MiaA and MiaE catalytic reactions, overview | Salmonella enterica |
metabolism | the enzyme is involved in the hypermodification enzyme pathway for 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37) synthesis. within the anticodon stem loop of tRNA, nucleotide modifications are frequently observed at position 34 (the wobble position) or position 37 (3' adjacent to the anticodon), nearly all eukaryotic and bacterial tRNAs that read codons starting with U are modified by the enzymes MiaA and MiaB and contain the ms2i6A modification | Salmonella enterica |
physiological function | enzyme MiaA catalyzes the nucleophilic substitution of the dimethylallyl group from dimethylallyl diphosphate (DMAPP) to the exocyclic 6-amino nitrogen of A37 (N6) to yield N6-isopentenyladenosine (i6A37) with release of inorganic diphosphate | Salmonella enterica |