Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli, recombinant overexpression of enzyme TRIT1 NIH 3T3 cells, recombinant overexpression of TRIT1 in HepG2 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D55G | site-directed mutagenesis, the mutant shows 50% reduced specific activity compared to the wild-type enzyme | Mus musculus |
additional information | knockdown of enzyme TRIT1 in NIH-3T3 cells by stable expression of shRNA. GPx1 expression is highly sensitive to tRNA[Ser]Sec modification in mouse liver expressing only tRNA[Ser]Sec carrying a 37A>G substitution, GPx1 levels in murine NIH-3T3 cells expressing the Trit1-knockdown construct are significantly reduced compared with control cells | Mus musculus |
T32A | site-directed mutagenesis, the mutant shows 90% reduced specific activity compared to the wild-type enzyme | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec) | Mus musculus | - |
diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q80UN9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
NIH-3T3 cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec) | - |
Mus musculus | diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec) | - |
? | |
dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec) | tRNA[Ser]Sec modification assay with [14C]DMAPP and in vitro transcribed tRNA[Ser]Sec. Incubation of in vitro transcribed tRNA[Ser]Sec with recombinant Trit1 transfers [14C]dimethylallyl diphosphate to tRNA[Ser]Sec reveals that 37A>G tRNA[Ser]Sec is resistant to isopentenylation by Trit1 | Mus musculus | diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dimethylallyl:tRNA[Ser]Sec transferase | - |
Mus musculus |
DMAPP:tRNA[Ser]Sec transferase | - |
Mus musculus |
TRIT1 | - |
Mus musculus |
tRNA([Ser]Sec)-isopentenyl transferase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of Trit1 reduces expression of selenoproteins. Trit1 proteins carrying the D55G or T32A mutations exhibit significantly reduced specific activity of 50% and 10%of wild-type respectively. Overexpression of TRIT1 does not increase selenoprotein expression in HepG2 cells | Mus musculus |
physiological function | mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression, full expression of selenoproteins requires modification of tRNA[Ser]Sec, including N6-isopentenylation of base A37 | Mus musculus |