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Literature summary for 2.5.1.75 extracted from
- N6-(delta-2-isopentenyl) adenosine. Biosynthesis in transfer ribonucleic acid in vitro (1969), Biochemistry, 8, 4361-4371.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Saccharomyces cerevisiae |  |
| EDTA | - |
Saccharomyces cerevisiae | |
| iodoacetamide | - |
Saccharomyces cerevisiae | |
| p-mercuribenzoate | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae | |
| Mg2+ | optimal concentration: 5 mM | Saccharomyces cerevisiae | |
| Mn2+ | can partially replace Mg2+ in activation | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + adenine37 in tRNA | Saccharomyces cerevisiae | biosynthesis of N6-(DELTA2-isopentenyl)adenosine which several species of tRNA contain adjacent to the 3'-end of the anticodon | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + adenine37 in tRNA | specific for DELTA2-isopentenyl phosphate | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
| dimethylallyl diphosphate + adenine37 in tRNA | no reaction with DELTA3-isopentenyl diphosphate | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
| dimethylallyl diphosphate + adenine37 in tRNA | no reaction with homologous native tRNA or homologous, permanganate-treated tRNA (permanganate specifically cleaves the DELTA2-isopentenyl groups of tRNA leaving adenosine residues) | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
| dimethylallyl diphosphate + adenine37 in tRNA | the enzyme from both yeast and rat liver catalyzes a significant incorporation of DELTA2-isopentenyl groups into untreated E. coli B tRNA | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
| dimethylallyl diphosphate + adenine37 in tRNA | biosynthesis of N6-(DELTA2-isopentenyl)adenosine which several species of tRNA contain adjacent to the 3'-end of the anticodon | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | pH 7: about 95% of maximum activity, pH 9.5: about 45% of maximum activity | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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