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Literature summary for 2.5.1.73 extracted from

  • Zhang, C.M.; Liu, C.; Slater, S.; Hou, Y.M.
    Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys) (2008), Nat. Struct. Mol. Biol., 15, 507-514.
    View publication on PubMed
Search for more literature for 2.5.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)-RIL cells Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-L-seryl-tRNACys + sulfide Methanocaldococcus jannaschii Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl–tRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNA–Cys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine L-cysteinyl-tRNACys + phosphate
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Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
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Methanocaldococcus jannaschii Q59072
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Purification (Commentary)

Purification (Comment) Organism
Q Sepharose FF column chromatography and FPLC Mono Q column chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-phospho-L-seryl-tRNACys + sulfide
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 Methanocaldococcus jannaschii L-cysteinyl-tRNACys + phosphate
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O-phospho-L-seryl-tRNACys + sulfide Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl–tRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNA–Cys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine Methanocaldococcus jannaschii L-cysteinyl-tRNACys + phosphate
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 ?
O-phospho-L-seryl-tRNACys + sulfide Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl–tRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNA–Cys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine. O-Phosphoseryl–tRNA synthetase and Sep-tRNA–Cys-tRNA synthase bind the reaction intermediate O-phospho-L-serine-tRNACys tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1a or infiltrating into the ribosome Methanocaldococcus jannaschii L-cysteinyl-tRNACys + phosphate
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Subunits

Subunits Comment Organism
More both SepRS and SepCysS are active as a monomer in theSepRS–SepCysS binary complex Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
Sep-tRNA-Cys-tRNA synthase both SepRS and SepCysS bind the reaction intermediate Sep-tRNACys tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1alpha or infiltrating into the ribosome Methanocaldococcus jannaschii
SepCysS
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 Methanocaldococcus jannaschii
SepCysS SepRS–SepCysS binary complex Methanocaldococcus jannaschii