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Literature summary for 2.5.1.73 extracted from

  • Yuan, J.; Sheppard, K.; Soell, D.
    Amino acid modifications on tRNA (2008), Acta Biochim. Biophys. Sin. (Shanghai), 40, 539-553.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
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Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-L-seryl-tRNACys + sulfide Methanococcus maripaludis the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential L-cysteinyl-tRNACys + phosphate
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?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
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-
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Archaeoglobus fulgidus O30207
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Methanococcus maripaludis
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-
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no activity in Methanobrevibacter smithii
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-
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no activity in Methanosphaera stadtmanae
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-phospho-L-seryl-tRNACys + sulfide
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Archaeoglobus fulgidus L-cysteinyl-tRNACys + phosphate
-
?
O-phospho-L-seryl-tRNACys + sulfide the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential Methanococcus maripaludis L-cysteinyl-tRNACys + phosphate
-
?
O-phospho-L-seryl-tRNACys + sulfide the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient Archaeoglobus fulgidus L-cysteinyl-tRNACys + phosphate
-
?
O-phospho-L-seryl-tRNACys + sulfide the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient Methanococcus maripaludis L-cysteinyl-tRNACys + phosphate
-
?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
Sep-tRNA:Cys-tRNA synthase
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Archaeoglobus fulgidus
Sep-tRNA:Cys-tRNA synthase
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Methanococcus maripaludis
SepCysS
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Archaeoglobus fulgidus
SepCysS
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Methanococcus maripaludis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
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Archaeoglobus fulgidus
pyridoxal 5'-phosphate
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Methanococcus maripaludis
pyridoxal 5'-phosphate dependent Archaeoglobus fulgidus