Cloned (Comment) | Organism |
---|---|
gene Mj1678 or pscS, functional complementation of a cysteinyl-tRNACys synthase mutant strain, phylogenetic analysis, expression in Escherichia coli | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the enzyme can synthesize Cys-tRNACys in a sepS deletion mutant when Na2S and O-phosphoserine are exogenously added, sepS encodes the O-phosphoseryl-tRNA synthetase, EC 6.1.1.B2 | Methanococcus maripaludis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-seryl-tRNACys + sulfide | Methanococcus maripaludis | the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism containing a dispensable cysteinyl-tRNACys synthase, EC 6.1.1.16, overview | L-cysteinyl-tRNACys + phosphate | - |
? | |
O-phospho-L-seryl-tRNACys + sulfide | Methanocaldococcus jannaschii | the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism lacking the cysteinyl-tRNACys synthase, EC 6.1.1.16, overview | L-cysteinyl-tRNACys + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
gene Mj1678 or pscS | - |
Methanococcus maripaludis | A4FWT8 | gene pcsS | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-seryl-tRNACys + sulfide | the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism containing a dispensable cysteinyl-tRNACys synthase, EC 6.1.1.16, overview | Methanococcus maripaludis | L-cysteinyl-tRNACys + phosphate | - |
? | |
O-phospho-L-seryl-tRNACys + sulfide | the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism lacking the cysteinyl-tRNACys synthase, EC 6.1.1.16, overview | Methanocaldococcus jannaschii | L-cysteinyl-tRNACys + phosphate | - |
? | |
O-phospho-L-seryl-tRNACys + sulfide | sulfide e.g. from Na2S, anaerobic reaction | Methanocaldococcus jannaschii | L-cysteinyl-tRNACys + phosphate | - |
? | |
O-phospho-L-seryl-tRNACys + sulfide | sulfide e.g. from Na2S, anaerobic reaction | Methanococcus maripaludis | L-cysteinyl-tRNACys + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SepCysS | - |
Methanocaldococcus jannaschii |
SepCysS | - |
Methanococcus maripaludis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Methanocaldococcus jannaschii | |
pyridoxal 5'-phosphate | - |
Methanococcus maripaludis |