Cloned (Comment) | Organism |
---|---|
gene N8DT1, recombinant expression in Solanum lycopersicum transgenic plants contitutively using CaMV 35S promoter | Sophora flavescens |
gene N8DT2, recombinant expression in Solanum lycopersicum transgenic plants contitutively using CaMV 35S promoter | Sophora flavescens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | production of prenylated flavonoids in Solanum lycopersicum as a host plant by means of metabolic engineering through recombinant overexpression of both the naringenin 8-dimethylallyltransferase (N8DT) gene, encoding a prenyltransferase from Sophora flavescens, and the chalcone isomerase (CHI) gene from Nicotiana tabacum cv. Samsun-NN, in transgenic tomato lines. Production of 8-dimethylallyl naringenin in the double transformants, while the production level is lower than N8DT single transformants. Tomato fruits overexpressing both N8DT and CHI genes accumulate high levels of rutin compared with wild-type tomato | Sophora flavescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the membrane-intrinsic enzyme protein contains a transit peptide sequence at the N-terminus that localizes it to the plastid even when expressed ectopically in heterologous host plants | Sophora flavescens | 9507 | - |
membrane | - |
Sophora flavescens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + (-)-(2S)-naringenin | Sophora flavescens | - |
diphosphate + sophoraflavanone B | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sophora flavescens | B1B3P3 | N8DT1; gene N8DT1 | - |
Sophora flavescens | B1B5P4 | N8DT2; gene N8DT2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + (-)-(2S)-naringenin | - |
Sophora flavescens | diphosphate + sophoraflavanone B | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N8DT1 | - |
Sophora flavescens |
N8DT2 | - |
Sophora flavescens |
naringenin 8-dimethylallyltransferase/chalcone isomerase | - |
Sophora flavescens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthetic pathway of prenylflavonoid and rutin | Sophora flavescens |
additional information | Tomato fruits overexpressing both N8DT and CHI genes accumulate high levels of rutin compared with wild-type tomato | Sophora flavescens |