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Literature summary for 2.5.1.7 extracted from

  • Kumar, S.; Parvathi, A.; Hernandez, R.L.; Cadle, K.M.; Varela, M.F.
    Identification of a novel UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Vibrio fischeri that confers high fosfomycin resistance in Escherichia coli (2009), Arch. Microbiol., 191, 425-429.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.7

Application

Application Comment Organism
drug development MurA will be a useful target to identify potential inhibitors of fosfomycin resistance in pharmacological studies Aliivibrio fischeri

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli with a 6-His tag Aliivibrio fischeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44700
-
 calculated from the 422 amino acids, confirmed by SDS-PAGE Aliivibrio fischeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Aliivibrio fischeri
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Aliivibrio fischeri B6DVJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
by metal affinity chromatography with a nickel column Aliivibrio fischeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Aliivibrio fischeri phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
MurA
-
 Aliivibrio fischeri
UDP-N-acetylglucosamine enolpyruvyl transferase
-
 Aliivibrio fischeri

pI Value

Organism Comment pI Value Maximum pI Value
Aliivibrio fischeri estimated
-
 5

General Information

General Information Comment Organism
physiological function key enzyme is involved in bacterial cell wall peptidoglycan synthesis and a target for the antimicrobial agent fosfomycin, a structural analog of the MurA substrate phosphoenol pyruvate Aliivibrio fischeri