Application | Comment | Organism |
---|---|---|
drug development | MurA will be a useful target to identify potential inhibitors of fosfomycin resistance in pharmacological studies | Aliivibrio fischeri |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli with a 6-His tag | Aliivibrio fischeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44700 | - |
calculated from the 422 amino acids, confirmed by SDS-PAGE | Aliivibrio fischeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Aliivibrio fischeri | - |
phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | B6DVJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
by metal affinity chromatography with a nickel column | Aliivibrio fischeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Aliivibrio fischeri | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurA | - |
Aliivibrio fischeri |
UDP-N-acetylglucosamine enolpyruvyl transferase | - |
Aliivibrio fischeri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aliivibrio fischeri | estimated | - |
5 |
General Information | Comment | Organism |
---|---|---|
physiological function | key enzyme is involved in bacterial cell wall peptidoglycan synthesis and a target for the antimicrobial agent fosfomycin, a structural analog of the MurA substrate phosphoenol pyruvate | Aliivibrio fischeri |