Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | included in the assay reaction mixture | Escherichia coli |
Application | Comment | Organism |
---|---|---|
medicine | target for development of antibacterial agents | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | binding study by isothermal titration calorimetry; binds covalently to Cys115 | Escherichia coli | |
PGE-553828 | competitive against UDP-GlcNAc; inhibition mechanism, kinetics | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli | |
0.0041 | - |
phosphoenolpyruvate | - |
Escherichia coli | |
0.0057 | - |
UDP-GlcNAc | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Escherichia coli | - |
MurA | - |
Escherichia coli MurA | - |
MurA | - |
Purification (Comment) | Organism |
---|---|
recombinant from overexpresssing strain | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli MurA | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.9 | - |
UDP-GlcNAc | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
PGE-553828 | - |
Escherichia coli |