Application | Comment | Organism |
---|---|---|
medicine | enzyme is a target for the antibiotic fosfomycin | Enterobacter cloacae |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, functional overexpression in Escherichia coli strain JM105 | Enterobacter cloacae |
Protein Variants | Comment | Organism |
---|---|---|
C115S | - |
Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | - |
Enterobacter cloacae | |
Trypsin | wild-type and mutant C115S, no protection via individually binding of substrates or inhibitor fosfomycin, but via binding of both, the 2 substrates and inhibitor fosfomycin | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44780 | - |
electrospray ionization-mass spectrometry | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | P33038 | MurA | - |
Enterobacter cloacae DSM 30054 | P33038 | MurA | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Enterobacter cloacae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | mechanism | Enterobacter cloacae | |
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | enyme exhibits an open conformation when substrate-free, and a closed, tightly-packed conformation upon substrate binding | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae DSM 30054 | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | structure | Enterobacter cloacae |