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Literature summary for 2.5.1.7 extracted from

  • Krekel, F.; Oecking, C.; Amrhein, N.; Macheroux, P.
    Substrate and inhibitor-induced conformational changes in the structurally related enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) (1999), Biochemistry, 38, 8864-8878.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme is a target for the antibiotic fosfomycin Enterobacter cloacae

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, functional overexpression in Escherichia coli strain JM105 Enterobacter cloacae

Protein Variants

Protein Variants Comment Organism
C115S
-
Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
fosfomycin
-
Enterobacter cloacae
Trypsin wild-type and mutant C115S, no protection via individually binding of substrates or inhibitor fosfomycin, but via binding of both, the 2 substrates and inhibitor fosfomycin Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44780
-
electrospray ionization-mass spectrometry Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae P33038 MurA
-
Enterobacter cloacae DSM 30054 P33038 MurA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Enterobacter cloacae

Reaction

Reaction Comment Organism Reaction ID
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine mechanism Enterobacter cloacae
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine enyme exhibits an open conformation when substrate-free, and a closed, tightly-packed conformation upon substrate binding Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
r
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Enterobacter cloacae DSM 30054 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
r

Subunits

Subunits Comment Organism
More structure Enterobacter cloacae