Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
expression in Escherichia coli | Corynebacterium glutamicum |
expression in Escherichia coli | Thermobifida fusca |
expression in Escherichia coli | Corynebacterium efficiens |
Protein Variants | Comment | Organism |
---|---|---|
I59A | change in product chain-length, produces prenyl products with chain length up to C50 | Thermobifida fusca |
I59F | inactive mutant enzyme | Thermobifida fusca |
I59M | change in product chain-length, produces prenyl products with chain length up to C50 | Thermobifida fusca |
I59V | change in product chain-length, produces prenyl products with chain length up to C50 | Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Thermobifida fusca | |
0.0023 | - |
geranyl diphosphate | pH 7.5, 37°C | Thermobifida fusca | |
0.0027 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Corynebacterium efficiens | |
0.0041 | - |
geranyl diphosphate | pH 7.5, 37°C | Corynebacterium efficiens | |
0.0045 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.0067 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Corynebacterium glutamicum | |
0.0068 | - |
geranyl diphosphate | pH 7.5, 37°C | Corynebacterium glutamicum | |
0.0078 | - |
geranyl diphosphate | pH 7.5, 37°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE, calculated from sequence | Corynebacterium glutamicum |
31000 | - |
x * 31000, SDS-PAGE, calculated from sequence | Thermobifida fusca |
31000 | - |
x * 31000, SDS-PAGE, calculated from sequence | Corynebacterium efficiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium efficiens | - |
- |
- |
Corynebacterium glutamicum | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Thermobifida fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
- |
Thermobifida fusca |
- |
Corynebacterium efficiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + isopentenyl diphosphate | only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate | Corynebacterium glutamicum | diphosphate + (2Z,6E)-farnesyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate | Mycobacterium tuberculosis | diphosphate + (2Z,6E)-farnesyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate | Thermobifida fusca | diphosphate + (2Z,6E)-farnesyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate | Corynebacterium efficiens | diphosphate + (2Z,6E)-farnesyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE, calculated from sequence | Corynebacterium glutamicum |
? | x * 31000, SDS-PAGE, calculated from sequence | Thermobifida fusca |
? | x * 31000, SDS-PAGE, calculated from sequence | Corynebacterium efficiens |
Synonyms | Comment | Organism |
---|---|---|
cis-farnesyl diphosphate synthase | - |
Corynebacterium glutamicum |
cis-farnesyl diphosphate synthase | - |
Mycobacterium tuberculosis |
cis-farnesyl diphosphate synthase | - |
Thermobifida fusca |
cis-farnesyl diphosphate synthase | - |
Corynebacterium efficiens |
cis-FPS | - |
Corynebacterium glutamicum |
cis-FPS | - |
Mycobacterium tuberculosis |
cis-FPS | - |
Thermobifida fusca |
cis-FPS | - |
Corynebacterium efficiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Corynebacterium glutamicum |
37 | - |
assay at | Mycobacterium tuberculosis |
37 | - |
assay at | Thermobifida fusca |
37 | - |
assay at | Corynebacterium efficiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
141 | - |
geranyl diphosphate | pH 7.5, 37°C | Corynebacterium efficiens | |
173 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Corynebacterium efficiens | |
229 | - |
geranyl diphosphate | pH 7.5, 37°C | Thermobifida fusca | |
282 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Thermobifida fusca | |
329 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Corynebacterium glutamicum | |
329 | - |
geranyl diphosphate | pH 7.5, 37°C | Corynebacterium glutamicum | |
788 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Mycobacterium tuberculosis | |
860 | - |
geranyl diphosphate | pH 7.5, 37°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Corynebacterium glutamicum |
7.5 | - |
assay at | Mycobacterium tuberculosis |
7.5 | - |
assay at | Thermobifida fusca |
7.5 | - |
assay at | Corynebacterium efficiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34390 | - |
geranyl diphosphate | pH 7.5, 37°C | Corynebacterium efficiens | |
48380 | - |
geranyl diphosphate | pH 7.5, 37°C | Corynebacterium glutamicum | |
49100 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Corynebacterium glutamicum | |
64070 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Corynebacterium efficiens | |
99570 | - |
geranyl diphosphate | pH 7.5, 37°C | Thermobifida fusca | |
110300 | - |
geranyl diphosphate | pH 7.5, 37°C | Mycobacterium tuberculosis | |
175100 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Mycobacterium tuberculosis | |
235000 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Thermobifida fusca |