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Literature summary for 2.5.1.68 extracted from
- Identification of a short (C15) chain Z-isoprenyl diphosphate synthase and a homologous long (C50) chain isoprenyl disphosphate synthase in Mycobacterium tuberculosis (2000), J. Biol. Chem., 275, 22876-22881.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Rv1086, DNA and amino acid sequence determination and anaylsis, overexpression in Mycobacterium smegmatis cytosol and membranes, the transformed cells show a 5fold increased enzyme activity | Mycobacterium tuberculosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Mycobacterium tuberculosis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-geranyl diphosphate + isopentenyl diphosphate | Mycobacterium tuberculosis | stereospecific reaction | (2Z,6E)-farnesyl diphosphate + diphosphate | - |
? | |
| (E)-geranyl diphosphate + isopentenyl diphosphate | Mycobacterium tuberculosis H37Rv | stereospecific reaction | (2Z,6E)-farnesyl diphosphate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00093 | - |
recombinant Rv1086 in cytosol of Mycobacterium smegmatis | Mycobacterium tuberculosis |
| 0.00097 | - |
recombinant Rv1086 in membranes of Mycobacterium smegmatis | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-geranyl diphosphate + isopentenyl diphosphate | stereospecific reaction | Mycobacterium tuberculosis | (2Z,6E)-farnesyl diphosphate + diphosphate | - |
? | |
| (E)-geranyl diphosphate + isopentenyl diphosphate | stereospecific reaction | Mycobacterium tuberculosis H37Rv | (2Z,6E)-farnesyl diphosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the enzyme contains a DD(XX)2D motif, secondary structure analysis | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Rv1086 | - |
Mycobacterium tuberculosis |
| short (C15) chain Z-isoprenyl diphosphate synthase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Mycobacterium tuberculosis |
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