Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta (DE3), sequencing | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Aeropyrum pernix | |
0.2 | - |
Sulfide | below, pH 6.7 | Aeropyrum pernix | |
28 | - |
O-acetyl-L-serine | pH 6.7 | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Aeropyrum pernix |
70580 | - |
sedimentation equilibrium ultracentrifugation | Aeropyrum pernix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aeropyrum pernix | biosynthesis of L-cysteine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant OASS | Aeropyrum pernix |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50.9 | - |
pH 6.7, 60°C, O-acetyl-L-serine sulfhydrylation | Aeropyrum pernix |
Storage Stability | Organism |
---|---|
4°C, enzyme solution, stable | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + dithiothreitol | OASS has a high activity in the L-cysteine desulfurization reaction | Aeropyrum pernix | S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + sulfide | - |
? | |
additional information | biosynthesis of L-cysteine | Aeropyrum pernix | ? | - |
? | |
additional information | enzyme with cystathionine beta-synthase and O-acetylserine sulfhydrylase activity in vitro, OASS has also L-serine sulfhydrylation and S-sulfo-L-cysteine synthesis activity | Aeropyrum pernix | ? | - |
? | |
O-acetyl-L-serine + sulfide | - |
Aeropyrum pernix | L-cysteine + acetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
APE1586 | Aeropyrum pernix K1, open reading frame | Aeropyrum pernix |
O-acetylserine sulfhydrylase | - |
Aeropyrum pernix |
OASS | - |
Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
6 h, pH 6.1 and 6.7: 10% loss of activity, pH 7.5: 44% loss of activity, pH 8.5: 89% loss of activity | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Aeropyrum pernix | |
202 | - |
O-acetyl-L-serine | pH 6.7 | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Aeropyrum pernix |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
6 h, 100°C, 10% loss of activity | Aeropyrum pernix |
6.7 | - |
6 h, 100°C, 10% loss of activity | Aeropyrum pernix |
7.5 | - |
6 h, 100°C, 44% loss of activity | Aeropyrum pernix |
8.5 | - |
6 h, 100°C, 89% loss of activity | Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | OASS contains 1.2 molecules pyridoxal 5'-phosphate per subunit, enzyme forms a Schiff base with pyridoxal 5'-phosphate | Aeropyrum pernix |