Cloned (Comment) | Organism |
---|---|
gene flA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) pLysS | Streptomyces cattleya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
S-adenosyl-L-methionine + fluoride | Streptomyces cattleya NBRC14057 | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cattleya | F8JPG4 | gene flA | - |
Streptomyces cattleya NBRC14057 | F8JPG4 | gene flA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold(DE3)pLysS by nickel affinity chromatography and gel filtration | Streptomyces cattleya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylaza-S-adenosyl-L-methionine + fluoride | optimal molar ratio of substrate to enzyme in this reaction equals to 112:1 for methylaza-S-adenosyl-L-methionine, modelling of methylaza-S-adenosyl-L-methionine bound in the active site using crystal structure with PDB ID 2V7U, reaction kinetics | Streptomyces cattleya | ? | - |
? | |
methylaza-S-adenosyl-L-methionine + fluoride | optimal molar ratio of substrate to enzyme in this reaction equals to 112:1 for methylaza-S-adenosyl-L-methionine, modelling of methylaza-S-adenosyl-L-methionine bound in the active site using crystal structure with PDB ID 2V7U, reaction kinetics | Streptomyces cattleya NBRC14057 | ? | - |
? | |
S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
S-adenosyl-L-methionine + fluoride | optimal molar ratio of substrate to enzyme in this reaction equals to 19:1 for S-adenosyl-L-methionine | Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya NBRC14057 | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
S-adenosyl-L-methionine + fluoride | optimal molar ratio of substrate to enzyme in this reaction equals to 19:1 for S-adenosyl-L-methionine | Streptomyces cattleya NBRC14057 | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-deoxy-5'-fluoroadenosine synthase | - |
Streptomyces cattleya |
fluorinase | - |
Streptomyces cattleya |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
assay at | Streptomyces cattleya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
recombinant enzyme with sbstrate methylaza-S-adenosyl-L-methionine | Streptomyces cattleya |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | activity range, profile overview | Streptomyces cattleya |