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Literature summary for 2.5.1.63 extracted from

  • Sergeev, M.; Morgia, F.; Javed, M.; Doi, M.; Keng, P.
    Enzymatic radiofluorination: fluorinase accepts methylaza-analog of SAM as substrate for FDA synthesis (2013), J. Mol. Catal. B, 97, 74-79.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene flA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) pLysS Streptomyces cattleya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + fluoride Streptomyces cattleya
-
5'-deoxy-5'-fluoroadenosine + L-methionine
-
?
S-adenosyl-L-methionine + fluoride Streptomyces cattleya NBRC14057
-
5'-deoxy-5'-fluoroadenosine + L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces cattleya F8JPG4 gene flA
-
Streptomyces cattleya NBRC14057 F8JPG4 gene flA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold(DE3)pLysS by nickel affinity chromatography and gel filtration Streptomyces cattleya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylaza-S-adenosyl-L-methionine + fluoride optimal molar ratio of substrate to enzyme in this reaction equals to 112:1 for methylaza-S-adenosyl-L-methionine, modelling of methylaza-S-adenosyl-L-methionine bound in the active site using crystal structure with PDB ID 2V7U, reaction kinetics Streptomyces cattleya ?
-
?
methylaza-S-adenosyl-L-methionine + fluoride optimal molar ratio of substrate to enzyme in this reaction equals to 112:1 for methylaza-S-adenosyl-L-methionine, modelling of methylaza-S-adenosyl-L-methionine bound in the active site using crystal structure with PDB ID 2V7U, reaction kinetics Streptomyces cattleya NBRC14057 ?
-
?
S-adenosyl-L-methionine + fluoride
-
Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
?
S-adenosyl-L-methionine + fluoride optimal molar ratio of substrate to enzyme in this reaction equals to 19:1 for S-adenosyl-L-methionine Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
?
S-adenosyl-L-methionine + fluoride
-
Streptomyces cattleya NBRC14057 5'-deoxy-5'-fluoroadenosine + L-methionine
-
?
S-adenosyl-L-methionine + fluoride optimal molar ratio of substrate to enzyme in this reaction equals to 19:1 for S-adenosyl-L-methionine Streptomyces cattleya NBRC14057 5'-deoxy-5'-fluoroadenosine + L-methionine
-
?

Synonyms

Synonyms Comment Organism
5'-deoxy-5'-fluoroadenosine synthase
-
Streptomyces cattleya
fluorinase
-
Streptomyces cattleya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
assay at Streptomyces cattleya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
recombinant enzyme with sbstrate methylaza-S-adenosyl-L-methionine Streptomyces cattleya

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 activity range, profile overview Streptomyces cattleya