Crystallization (Comment) | Organism |
---|---|
purified enzyme in complex with 5',5'-difluorodeoxy-2-ethynyladenosine and tartrate, X-ray diffraction structure determination and analysis at 1.8 A resolution, molecular replacement using structure with PDB ID 1RQR | Streptomyces cattleya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cattleya | Q70GK9 | gene flA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-deoxy-5'-fluoroadenosine + L-selenomethionine | - |
Streptomyces cattleya | Se-adenosyl-L-selenomethionine + fluoride | - |
r | |
additional information | the enzyme binds 5',5'-dideoxy-5'-fluoroadenosine with similar affinity as 5'-deoxy-5'-fluoroadenosine, but does not show formation of Se-adenosyl-L-selenomethionine with added L-selenomethionine as it does with 5'-deoxy-5'-fluoroadenosine. The difluoromethyl group bridges interactions that are essential for activation of the single fluorine in 5'-deoxy-5'-fluoroadenosine. An unusual hydrogen bonding interaction between the hydrogen of the difluoromethyl group and one of the hydroxyl oxygens of the tartrate ligand is also observed. The bridging interactions, coupled with the inherently stronger C-F bond in the difluoromethyl group, offers an explanation for why no reaction is observed | Streptomyces cattleya | ? | - |
? | |
S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
fluorinase | - |
Streptomyces cattleya |