Literature summary for 2.5.1.63 extracted from
Iwai, N.; Tanaka, T.; Kitazume, T.
Utility of ionic liquid for improvement of fluorination reaction with immobilized fluorinase (2009), J. Mol. Catal. B, 59, 131-133.
No PubMed abstract available
Application
Application |
Comment |
Organism |
biotechnology |
the rate of the enzymatic fluorination reaction can be enhanced by using ionic liquids and immobilizing the enzyme with a water-absorbing polymer which stabilizes the enzyme, 1-octyl-3-methylimidazolium hexafluorophosphate and 1-hexyl-3-methylimidazolium hexafluorophosphate raise the conversion yield 2.4times or 1.6times compared to Tris-HCl buffer, pH 8.0, 1-hexyl-3-methylimidazolium tetrafluoroborate, 1-octyl-3-methylimidazolium tetrafluoroborate, 1-octyl-3-methylimidazolium hexafluorophosphate, and 1-hexyl-3-methylimidazolium hexafluorophosphate increase total conversation yields 2.2times to 4times more in immobilized compared to non-immobilized enzyme |
Streptomyces cattleya |
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression of His-tagged enzyme in Escherichia coli BL21(DE3) pLysS with plasmid pET28b+ |
Streptomyces cattleya |
General Stability
General Stability |
Organism |
water stabilizes the enzyme, thus water-absorbing polymer and ionic liquid is used to immobilize the enzyme and keep it surrounded by water |
Streptomyces cattleya |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
S-adenosyl-L-methionine + fluoride |
Streptomyces cattleya |
20 mg water-absorbing polymer in ionic liquid or 50 mM Tris-HCl buffer, pH 8.0, 50 nM S-adenosyl-L-methionine, 4 mircoM KF, 37°C, exteraction of product 5'-deoxy-5'-fluoroadenosine with diethyl ether |
5'-deoxy-5'-fluoroadenosine + L-methionine |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Streptomyces cattleya |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
nickel-affinity column |
Streptomyces cattleya |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
S-adenosyl-L-methionine + fluoride |
20 mg water-absorbing polymer in ionic liquid or 50 mM Tris-HCl buffer, pH 8.0, 50 nM S-adenosyl-L-methionine, 4 mircoM KF, 37°C, exteraction of product 5'-deoxy-5'-fluoroadenosine with diethyl ether |
Streptomyces cattleya |
5'-deoxy-5'-fluoroadenosine + L-methionine |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
adenosyl-fluoride synthase |
- |
Streptomyces cattleya |