Cloned (Comment) | Organism |
---|---|
gene hemC, a single gene for PBGD in chromosome 5, recombinant expression of a codon-optimized PBGD gene in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme with bound cofactor, crystallization in the dark due to light-sensitivity of the cofactor, hanging drop method, mixing of 5 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 5 mM DTT, with reservoir solution containing 25% w/v PEG 4000, 100 mM sodium citrate, pH 5.6, and 200 mM ammonium sulfate, X-ray diffraction structure determination and analysis at 1.45 A resolution, molecular modelling | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | in the chloroplast, the precursor is processed by the removal of an N-terminal transit peptide which is around 60 residues in length | Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen + H2O | Arabidopsis thaliana | - |
hydroxymethylbilane + 4 NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q43316 | gene hemC | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3 | four molecules of the pyrrole porphobilinogen are condensed to form the linear tetrapyrrole preuroporphyrinogen (hydroxymethylbilane). Hydrolysis of the linkage between the first substrate moiety and the cofactor releases the tetrapyrrole product preuroporphyrinogen | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen + H2O | - |
Arabidopsis thaliana | hydroxymethylbilane + 4 NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure analysis, molecular modelling, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
PBGD | - |
Arabidopsis thaliana |
porphobilinogen deaminase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dipyrromethane | the active site possesses the unusual dipyrromethane cofactor which is extended during the reaction by the sequential addition of the four substrate molecules. The cofactor is linked covalently to the enzyme through a thioether bridge to the invariant Cys254, binding structure, overview. Hydrolysis of the linkage between the first substrate moiety and the cofactor releases the tetrapyrrole product preuroporphyrinogen. The dipyrromethane cofactor of enzyme PBGD is light-sensitive | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme hydroxymethylbilane synthase catalyses a key early step of the heme and chlorophyll biosynthesis pathways in which four molecules of the monopyrrole porphobilinogen are condensed to form a linear tetrapyrrole | Arabidopsis thaliana |